Entry of coxsackievirus A9 into host cells: specific interactions with alpha v beta 3 integrin, the vitronectin receptor

Virology
M RoivainenT Hyypiä

Abstract

Attachment and entry of coxsackievirus A9 (CAV-9) to GMK cells were previously shown to be dependent on an arginine-glycine-aspartic acid (RGD) motif in the capsid protein VP1, suggesting integrins as candidate receptors for the virus. We have pursued the matter further and show that antibodies specific for the alpha v and/or beta 3 integrin subunits protect GMK cells from CAV-9 infection. Affinity purification of radioiodinated cell surface proteins using CAV-9 or virus-specific peptide (RRRGDL) columns confirmed that the alpha v beta 3 heterodimer, known as the vitronectin receptor, is recognized by the virus in GMK cells. Other proteins, of lower molecular weight (less than 40 kDa), were also bound to and specifically eluted from the columns, but their possible role in attachment and entry of CAV-9 remains to be elucidated by further studies. Of several other related viruses studied, only echovirus 22, which also has an RGD motif in the VP1 capsid protein, was found to compete for cell surface binding with CAV-9.

Citations

Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·E RiederP W Mason
Oct 24, 2008·Phytopathology·M KikkertR Goldbach
Apr 16, 2004·Clinical Microbiology Reviews·Marvin J Grubman, Barry Baxt
Feb 1, 2013·Journal of Virology·Shabih ShakeelSarah J Butcher
Jun 14, 2000·Journal of Virology·K TriantafilouN Fernandez
Sep 18, 2001·Journal of Virology·Y BoonyakiatG Stanway
Feb 13, 2001·Journal of Virology·P Joki-KorpelaT Hyypiä
Jan 5, 2002·Journal of Virology·Terry JacksonAndrew M Q King
Jan 19, 2002·Journal of Virology·Varpu MarjomäkiJyrki Heino
Jun 15, 2004·Journal of Virology·Ciğdem H WilliamsGlyn Stanway
Nov 16, 2004·Journal of Virology·Anna E WhitfieldThomas L German
Jun 16, 2005·Journal of Virology·Vivian O'DonnellBarry Baxt
Jan 1, 1996·Annual Review of Cell and Developmental Biology·E Ruoslahti
Aug 5, 2005·Annual Review of Phytopathology·Anna E WhitfieldThomas L German
Jul 12, 2007·Virology Journal·Nidia H De Jesus
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·C A GuerreroC F Arias
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·I N GavrilovskayaE R Mackow
Nov 21, 2009·The EMBO Journal·Johanna JokinenJyrki Heino
May 10, 2013·The Journal of General Virology·Katja SchmidtMartin H Groschup
Dec 18, 2008·The Journal of General Virology·Outi HeikkiläTimo Hyypiä
Dec 12, 2012·Advances in Virology·Pirjo MerilahtiPetri Susi
Jul 21, 2005·Technology in Cancer Research & Treatment·Reetta RiikonenChristian Oker-Blom
Apr 17, 1999·Molecular Reproduction and Development·R A BronsonD M Phillips
Feb 26, 2016·Viruses·Jani Ylä-PeltoPetri Susi
Feb 3, 2018·International Journal of Molecular Sciences·Bryce LaFoyaAllan R Albig
Jul 15, 2015·Journal of Virology·Shabih ShakeelSarah J Butcher
Feb 28, 2014·Journal of Virology·Moona HuttunenVarpu Marjomäki
Feb 8, 2005·The Journal of Biological Chemistry·Boyd Butler, Scott D Blystone
Sep 21, 2000·Folia Microbiologica·L Jindrák, L Grubhoffer
Jan 1, 1996·Archives of Virology·M RoivainenT Hovi

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