Entry of Spiroplasma citri into Circulifer haematoceps cells involves interaction between spiroplasma phosphoglycerate kinase and leafhopper actin.

Applied and Environmental Microbiology
Fabien LabroussaaColette Saillard

Abstract

Transmission of the phytopathogenic mollicutes, spiroplasmas, and phytoplasmas by their insect vectors mainly depends on their ability to pass through gut cells, to multiply in various tissues, and to traverse the salivary gland cells. The passage of these different barriers suggests molecular interactions between the plant mollicute and the insect vector that regulate transmission. In the present study, we focused on the interaction between Spiroplasma citri and its leafhopper vector, Circulifer haematoceps. An in vitro protein overlay assay identified five significant binding activities between S. citri proteins and insect host proteins from salivary glands. One insect protein involved in one binding activity was identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) as actin. Confocal microscopy observations of infected salivary glands revealed that spiroplasmas colocated with the host actin filaments. An S. citri actin-binding protein of 44 kDa was isolated by affinity chromatography and identified by LC-MS/MS as phosphoglycerate kinase (PGK). To investigate the role of the PGK-actin interaction, we performed competitive binding and internalization assays on leafhopper cultured cell lines (Ciha-1) in which ...Continue Reading

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Citations

Mar 24, 2016·BMC Microbiology·Marie-Pierre DubranaColette Saillard
Nov 18, 2014·Biochemical Society Transactions·Brian Henderson
Aug 25, 2016·Frontiers in Plant Science·Laura M Perilla-Henao, Clare L Casteel
Jan 25, 2018·Current Microbiology·Guo-Dong ZhaHan-Shou Yu

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