PMID: 8588102Nov 1, 1995Paper

Entry of the bacterium ileal symbiont intracellularis into cultured enterocytes and its subsequent release

Research in Veterinary Science
S McOristG H Lawson

Abstract

Separate suspensions of two strains of ileal symbiont (IS) intracellularis, an obligate intracellular bacterium and the causative agent of porcine proliferative enteropathy, were added to 40 or 80 per cent confluent monolayers of established cultures of rat (IEC-18) or pig enterocytes (IPEC-J2). Peak numbers of intracellular organisms were detected within the enterocytes six days later, but no cytopathic effects were evident. After an initial close association with the cell membrane of the enterocytes, single bacteria were internalised after three hours within membranes-bound vacuoles. The formation of an electron-dense projection between cell membranes and external bacteria was only evident if the bacterial suspensions were centrifuged on to the monolayers. The release of internalised bacteria into the cytoplasm, with the breakdown and loss of membrane-bound vacuoles, was also evident three hours after infection. Internalised bacteria were associated with, but not observed within, coated membrane pits. Mitochondria were closely associated with internalised vacuoles and with released bacteria. Two to six days after infection, multiplication of the bacteria free in the cytoplasm was frequently observed. In infected cells six day...Continue Reading

References

May 1, 1989·Veterinary Pathology·S McOristN MacIntyre
Feb 1, 1972·Antimicrobial Agents and Chemotherapy·Y Becker, Y Asher
Mar 1, 1994·Research in Veterinary Science·S JasniG H Lawson

❮ Previous
Next ❯

Citations

Aug 17, 2001·Veterinary Microbiology·D G Smith, G H Lawson
Mar 1, 1997·Veterinary Microbiology·S McOristD G Smith
Apr 6, 2006·Animal Health Research Reviews·Jeremy J KrollD L Hank Harris
Feb 26, 2010·Acta Veterinaria Scandinavica·Torsten S BoutrupTim K Jensen
Feb 24, 2000·Journal of Comparative Pathology·G H Lawson, C J Gebhart
Oct 11, 2014·The Veterinary Clinics of North America. Equine Practice·Allen E PageDavid W Horohov
Feb 20, 2010·Journal of Comparative Pathology·T S BoutrupT K Jensen
May 13, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·T K JensenM Boye
Jan 31, 2014·Veterinary Pathology·F A Vannucci, C J Gebhart
Mar 13, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mary DrozdGireesh Rajashekara
Jun 26, 2003·Veterinary Pathology·N MacIntyreS M Rhind
Dec 9, 1998·Equine Veterinary Journal·D G Smith
Oct 11, 2003·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·M DittmarM M Wittenbrink
Aug 1, 2020·PloS One·Carlos Eduardo Real PereiraRoberto Mauricio Carvalho Guedes
Jun 18, 2017·Veterinary Pathology·Roberto M C GuedesConnie J Gebhart
Jul 22, 2019·Veterinary Research·Talita Pilar ResendeConnie Gebhart
Nov 30, 2018·Frontiers in Physiology·Sarah C PearceKenneth Racicot

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.