Entry of Trypanosoma brucei gambiense into microvascular endothelial cells of the human blood-brain barrier

International Journal for Parasitology
Olga V NikolskaiaDennis J Grab

Abstract

Using an in vitro model of the human blood-brain barrier consisting of human brain microvascular endothelial cells we recently demonstrated that Trypanosoma brucei gambiense bloodstream-forms efficiently cross these cells via a paracellular route while Trypanosoma brucei brucei crosses these cells poorly. Using a combination of techniques that include fluorescence activated cell sorting, confocal and electron microscopy, we now show that some T.b. gambiense blood stream form parasites have the capacity to enter human brain microvascular endothelial cells. The intracellular location of the trypanosomes was demonstrated in relation to the endothelial cell plasma membrane and to the actin cytoskeleton. These parasites may be a terminal stage within a lysosomal compartment or they may be viable trypanosomes that will be able to exit the brain microvascular endothelial cells. This process may provide an additional transcellular route by which the parasites cross the blood-brain barrier.

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Nov 26, 2004·The Journal of Parasitology·Dennis J GrabKwang Sik Kim
Jan 25, 2005·Infection and Immunity·Dennis J GrabKwang Sik Kim

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Citations

Dec 24, 2009·Parasitology·Jean Rodgers
Jul 7, 2009·Annual Review of Microbiology·Katherine S RalstonKent L Hill
Sep 26, 2006·The Journal of Clinical Investigation·Olga V NikolskaiaDennis J Grab
Jun 22, 2012·PLoS Neglected Tropical Diseases·Vincent JamonneauBruno Bucheton
Nov 30, 2011·Molecular and Biochemical Parasitology·Richard T KangetheTheresa H T Coetzer
Mar 10, 2009·Journal of Neuroimmunology·Jean Rodgers
Jun 7, 2015·Current Opinion in Microbiology·Norikiyo Ueno, Melissa B Lodoen
Mar 18, 2006·International Journal for Parasitology·Kwang Sik Kim
May 9, 2012·Journal of Microencapsulation·Ian G TuckerHimang Mujoo
Mar 27, 2010·FEMS Microbiology Reviews·Hany M Elsheikha, Naveed Ahmed Khan
Mar 31, 2012·Virulence·Willias Masocha, Krister Kristensson
May 2, 2014·Trends in Neurosciences·Marina Bentivoglio, Krister Kristensson
Oct 7, 2009·Nature Reviews. Microbiology·Mark C Field, Mark Carrington
Mar 5, 2019·Cellular Microbiology·Mariana De NizFriedrich Frischknecht
Jul 8, 2019·Trends in Parasitology·Thibault AllainAndre G Buret
Jan 23, 2021·RSC Medicinal Chemistry·Anutthaman Parthasarathy, Karunakaran Kalesh

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