Aug 4, 2012

Entry tropism of BK and Merkel cell polyomaviruses in cell culture

PloS One
Rachel M SchowalterChristopher B Buck

Abstract

Merkel Cell Polyomavirus (MCV or MCPyV) was recently discovered in an aggressive form of skin cancer known as Merkel cell carcinoma (MCC). Integration of MCV DNA into the host genome likely contributes to the development of MCC in humans. MCV infection is common and many healthy people shed MCV virions from the surface of their skin. MCV DNA has also been detected in samples from a variety of other tissues. Although MCC tumors serve as a record that MCV can infect the Merkel cell lineage, the true tissue tropism and natural reservoirs of MCV infection in the host are not known. In an effort to gain insight into the tissue tropism of MCV, and to possibly identify cellular factors responsible for mediating infectious entry of the virus, the infection potential of human cells derived from a variety of tissues was evaluated. MCV gene transfer vectors (pseudoviruses) carrying reporter plasmid DNA encoding GFP or luciferase genes were used to transduce keratinocytes and melanocytes, as well as lines derived from MCC tumors and the NCI-60 panel of human tumor cell lines. MCV transduction was compared to transduction with pseudoviruses based on the better-studied human BK polyomavirus (BKV). The efficiency of MCV and BKV transduction o...Continue Reading

  • References58
  • Citations32

Mentioned in this Paper

Malignant Neoplasm of Skin
Tumor Cells, Uncertain Whether Benign or Malignant
Flow Cytometry
GBA wt Allele
Microarray Analysis
Melanocyte
PLXNA1 gene
Urine
T-Lymphocyte
Transfection

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