Enumeration of aromatic oxygenase genes to evaluate biodegradation during multi-phase extraction at a gasoline-contaminated site

Journal of Hazardous Materials
Brett R BaldwinLoring Nies

Abstract

Multi-phase extraction (MPE) is commonly used at petroleum-contaminated sites to volatilize and recover hydrocarbons from the vadose and saturated zones in contaminant source areas. Although primarily a physical treatment technology, the induced subsurface air flow can potentially increase oxygen supply and promote aerobic biodegradation of benzene, toluene, ethylbenzene, and xylenes (BTEX), the contaminants of concern at gasoline-contaminated sites. In this study, real-time PCR enumeration of aromatic oxygenase genes and PCR-DGGE profiles were used to elucidate the impact of MPE operation on the aquifer microbial community structure and function at a gasoline-contaminated site. Prior to system activation, ring-hydroxylating toluene monooxygenase (RMO) and naphthalene dioxygenase (NAH) gene copies were on the order of 10(6) to 10(10)copies L(-1) in groundwater samples obtained from BTEX-impacted wells. Aromatic oxygenase genes were not detected in groundwater samples obtained during continuous MPE indicating decreased populations of BTEX-utilizing bacteria. During periods of pulsed MPE, total aromatic oxygenase gene copies were not significantly different than prior to system activation, however, shifts in aromatic catabolic ge...Continue Reading

References

Jan 1, 1996·Applied Biochemistry and Biotechnology·D P Chandler, F J Brockman
Oct 23, 1997·Bulletin of Environmental Contamination and Toxicology·K L Shaffer, C G Uchrin
Jan 22, 2003·Journal of Applied Microbiology·M ZucchiD Daffonchio
Jun 6, 2003·Applied and Environmental Microbiology·Brett R BaldwinLoring Nies
Dec 8, 2005·Applied and Environmental Microbiology·Cindy H NakatsuAllan Konopka

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Citations

Dec 20, 2011·Environmental Science & Technology·Gregory H LefevreRaymond M Hozalski
Dec 22, 2009·Applied and Environmental Microbiology·Weidong Kong, Cindy H Nakatsu
Apr 16, 2013·Environmental Microbiology Reports·Michael LarentisTillmann Lueders
Nov 4, 2010·FEMS Microbiology Ecology·Cécile MilitonPierre Peyret
Aug 5, 2010·Environmental Science & Technology·Brett R BaldwinDora Ogles

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