Enumeration of CD34-positive Stem Cells Using the ADAMII Image-based Fluorescence Cell Counter

Annals of Laboratory Medicine
Haein YuYonggoo Kim

Abstract

It is very important to accurately enumerate CD34-positive (CD34+) cells for successful hematopoietic stem cell transplantation (HSCT). We evaluated the ability of the newly developed image based-immunofluorescence cell counter ADAMII (NanoEntek, Seoul, Korea) to enumerate CD34+ cells, which was improved through simultaneous CD45 analysis. We enumerated CD34+ cells with ADAMII using 19 peripheral blood (PB) and 91 leukapheresis samples from HSCT donors. Analytical performance, including precision and linearity, was analyzed, and sample stability during storage was evaluated. Viable CD34+ cell count (vCD34) and viable CD45+ cell count (vCD45) and the percentage of viable CD34+ cells among viable CD45+ cells (CD34/CD45) as measured by ADAMII were compared with the corresponding values from two flow cytometry assays, using regression analysis. ADAMII demonstrated acceptable precision, as CV values of vCD34 from six samples with different counts were all <10% (range: 3.49-9.51%). CV values of the vCD45 and CD34/45 ranged from 4.03% to 9.67% and from 2.48% to 10.07%, respectively. The linearity of vCD34 showed an excellent R² value (0.99) when analyzed using the intended count and flow cytometry data. The ADAMII and two flow cytomet...Continue Reading

Citations

Aug 23, 2021·Analytical Biochemistry·Dimitrina KrastevaTzonka Godjevargova

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Methods Mentioned

BETA
leukapheresis
flow
leukaphersis
flow cytometry

Software Mentioned

BD FACSDiva
ADAMII
BD FACSCanto clinical
NanoEntek
FACSAria
MedCalc

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