Enumeration of mycoplasmas after acridine orange staining.

Applied and Environmental Microbiology
S Rosendal, A Valdivieso-Garcia

Abstract

Samples of liquid mycoplasma cultures were mixed with equal part of a 0.01% solution of acridine orange and placed on agar plates. The number of fluorescing organisms per field was counted in an epifluorescence microscope at an X 1,000 magnification. When the number of fluorescing organisms per field was related to the number of colony-forming units per milliliter during the growth cycle, highly significant correlation was found in cultures with greater than or equal to 10(6) colony-forming units per ml during the exponential growth phase. The counts were weakly correlated during the stationary phase and not correlated during the death phase. This technique provides a mean to enumerate mycoplasmas in liquid cultures.

References

Sep 1, 1966·Applied Microbiology·D E Jasper, N C Jain
Mar 1, 1972·The Journal of Applied Bacteriology·R S Windsor, C D Boarer
Nov 23, 1956·Science·F MASINL VON BERTALANFFY

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Citations

Jan 26, 2008·Animal Health Research Reviews·Jeff L Caswell, Marie Archambault
Oct 1, 1984·Journal of Clinical Microbiology·D E JasperD A Barnum
Apr 1, 1984·Journal of Clinical Microbiology·R G Cluss, N L Somerson
Jul 1, 1983·Infection and Immunity·S J Geary, E M Walczak

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