PMID: 14607402Nov 11, 2003

Enumeration of water-borne bacteria using viability assays and flow cytometry: a comparison to culture-based techniques

Journal of Microbiological Methods
D HoefelC P Saint


Maintaining optimal conditions in catchments or distribution systems relies heavily on water authorities having access to rapid and accurate water quality data, including an indication of bacteriological quality. In this study, the BacLight bacterial viability kit and carboxyfluorescein diacetate (CFDA) were coupled with flow cytometry (FCM) for rapid detection of physiologically active bacteria from raw and potable waters taken from various locations around South Australia. Results were compared to the direct viable count (DVC) and quantitative DVC (qDVC), in addition to the culture-based methods of the heterotrophic plate count (HPC) and a commercial SimPlate technique. Raw and potable water analysis revealed that DVC and culture-based techniques reported significantly fewer viable bacteria compared to the number of physiologically active bacteria detected using the rapid FCM assays, where this difference appeared to be nonlinear across different samples. Inconclusive results were obtained using qDVC as a viability assay. In particular, HPC results were 2-4 log orders of magnitude below that reported by the FCM assays for raw waters. Few bacteria in potable waters examined were culturable by HPC, even though FCM assays report...Continue Reading


Sep 9, 2000·FEMS Microbiology Reviews·J Vives-RegoG Nebe-von Caron
Sep 15, 2000·Journal of Bacteriology·M EricssonT Nyström
Feb 13, 2001·Journal of Microbiological Methods·R RamalhoP A Gibbs


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Sep 12, 2007·Antimicrobial Agents and Chemotherapy·Woong Sik JangIn Hee Lee
Oct 22, 2008·Applied and Environmental Microbiology·Bryan P TracyEleftherios T Papoutsakis
Feb 5, 2008·Applied and Environmental Microbiology·Woo Kyung JungYong Ho Park
Mar 24, 2009·Applied and Environmental Microbiology·B C Ferrari, M R Gillings
Feb 5, 2005·Applied and Environmental Microbiology·Knut RudiAskild L Holck
Dec 6, 2006·Infection and Immunity·Rosanna CapparelliDomenico Iannelli
Aug 2, 2007·Canadian Journal of Microbiology·M-H PheJ-C Block
Apr 2, 2010·BMC Microbiology·Suzanne T ReadK Rabaey
Jan 14, 2012·Biointerphases·John-Bruce D GreenMark A Nordhaus
Dec 18, 2013·Water Research·Eric P HolingerNorman R Pace
Oct 14, 2008·Applied and Environmental Microbiology·Séverine AllegraSerge Riffard
Feb 13, 2016·Frontiers in Microbiology·Emmanuelle I PrestJ S Vrouwenvelder
Apr 29, 2015·The Science of the Total Environment·J BridgemanJoby B Boxall
Aug 7, 2010·Water Research·Thomas Egli
Jun 15, 2010·Trends in Biotechnology·Yingying WangNico Boon
Nov 8, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Irene O L McHugh, Ayly Ling Tucker
May 22, 2008·Journal of Applied Microbiology·G HallasW L Grooby
Apr 11, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·F HammesBernhard Sonnleitner
May 3, 2015·International Journal of Biological Macromolecules·Haibo FengJuan Liu
Jan 26, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Hans Peter FüchslinThomas Egli
May 23, 2015·FEMS Microbiology Reviews·Mark A LeverBo Barker Jørgensen
Jan 13, 2005·Letters in Applied Microbiology·K NakajimaM Nasu
May 27, 2008·Cytometry. Part B, Clinical Cytometry·Tania FalcioniWally Baffone
May 20, 2015·Journal of the Science of Food and Agriculture·Xi DengZhenqiang Chen
May 5, 2011·Biotechnology Advances·R Lencastre FernandesK V Gernaey
Jan 27, 2015·Environmental Science & Technology·Allison M TruhlarTammo S Steenhuis
Sep 23, 2014·Journal of Hazardous Materials·P FoladoriS Tamburini
May 9, 2014·ACS Applied Materials & Interfaces·Haitao WangChongzheng Na
Apr 2, 2013·The Science of the Total Environment·Dipok Chandra SarkerAnna Heitz
Oct 30, 2016·PloS One·Emmanuelle I PrestJ S Vrouwenvelder
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