ENV7 and YCK3, which encode vacuolar membrane protein kinases, genetically interact to impact cell fitness and vacuole morphology

FEMS Yeast Research
Surya P Manandhar, Editte Gharakhanian

Abstract

Saccharomyces cerevisiae vacuoles serve as a model for membrane fusion and fission. Yck3, a vacuolar membrane kinase, has been implicated in regulation of vacuole fusion. Recently, we established Env7 as another vacuolar membrane protein kinase with similar but nonredundant function to Yck3. Here, we report that native Env7 localizes to the vacuole independent of Yck3, where as its phosphorylation is YCK3 dependent. We also show that env7Δyck3Δ double mutant exhibits severely compromised fitness, altered cell size and bud vacuoles, and F-class vacuolar morphology. Our results establish negative genetic interactions between ENV7 and YCK3 and suggest cooperative roles for the two conserved genes in regulation of membrane dynamics. Such genetic buffering supports a critical role for membrane flux in global cell fitness.

References

May 1, 1986·The Journal of Cell Biology·T H StevensR Schekman
Mar 1, 1995·The Journal of Cell Biology·T A Vida, S D Emr
Oct 13, 2001·Molecular Biology of the Cell·J V Reddy, M N Seaman
Jul 27, 2002·Annual Review of Cell and Developmental Biology·James Shorter, Graham Warren
Oct 18, 2002·Current Opinion in Cell Biology·Francis A Barr
Nov 20, 2004·Cell·Christopher PetersAndreas Mayer
Feb 3, 2005·The Journal of Cell Biology·Tracy J LaGrassa, Christian Ungermann
May 26, 2005·Biochimica Et Biophysica Acta·Katherine Bowers, Tom H Stevens
Mar 28, 2006·The Journal of Biological Chemistry·Bridget L Decker, William T Wickner
Jul 27, 2007·Molecular Biology of the Cell·Tonie L BaarsAndreas Mayer
Oct 13, 2007·Autophagy·Clemens W OstrowiczChristian Ungermann
Sep 13, 2008·Biochimica Et Biophysica Acta·Sheena Claire Li, Patricia M Kane
Sep 24, 2008·The Journal of Cell Biology·Christopher L BrettAlexey J Merz
Feb 6, 2009·Molecular Biology of the Cell·Margarita CabreraChristian Ungermann
May 4, 2010·Cell·Margarita Cabrera, Christian Ungermann
Aug 6, 2010·Trends in Cell Biology·John Armstrong
Nov 9, 2010·Current Biology : CB·Cornelia BröckerChristian Ungermann
Nov 30, 2010·Current Opinion in Biotechnology·Michael CostanzoCharles Boone
Dec 14, 2012·Cell Division·Huzefa DungrawalaBrandt L Schneider

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