Envelope instability in DNA adenine methylase mutants of Salmonella enterica

Microbiology
M G PucciarelliF García-Del Portillo

Abstract

Mutants of Salmonella enterica serovar Typhimurium lacking DNA adenine (Dam) methylase show reduced secretion of invasion effectors encoded in the Salmonella-pathogenicity island 1 (SPI-1). Concomitant with this alteration, a high number and quantity of extracellular proteins are detected in cultures of Dam(-) mutants. This study shows by subcellular fractionation analysis that the presence of numerous extracellular proteins in cultures of Dam(-) mutants is linked to an exacerbated release of membrane particulate material. The membrane 'leaky' phenotype and the impaired functionality of type III secretion systems were, however, unrelated since exacerbated release of proteins to the medium was evident in Dam(-) strains carrying mutations in either SPI-1 (invA, invJ) or flagellar (flhD) genes. This result supports the view that Dam methylation controls a plethora of cellular processes. Electron microscopy analysis demonstrated that the accumulation of membrane particulate material occurs preferentially as vesicles in stationary cultures of Dam(-) strains. In addition, a reduction in the relative amount of peptidoglycan-associated lipoprotein (PAL), TolB, OmpA and murein lipoprotein (Lpp) bound to peptidoglycan was observed in act...Continue Reading

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Citations

Oct 16, 2015·Molecular Microbiology·Gadea Rico-PérezFrancisco García-del Portillo
Aug 27, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kai PapenfortJörg Vogel
Jun 30, 2012·International Journal of Antimicrobial Agents·Ming-Han TsaiCheng-Hsun Chiu
Sep 25, 2004·Molecular Microbiology·Gustavo Domínguez-BernalFrancisco García-del Portillo
Oct 1, 2011·Environmental Microbiology Reports·Andreas J BäumlerJosep Casadesús
Mar 22, 2016·Nature Genetics·Nadia R CohenJames J Collins
Jul 14, 2004·Research in Microbiology·Thomas HenryRoland Lloubès
Sep 19, 2009·Journal of Medical Microbiology·Megan E Merritt, Janet R Donaldson
May 3, 2008·Microbiology·Javier F Mariscotti, Francisco García-Del Portillo
Apr 26, 2014·Environmental Microbiology·Sara B HernándezJosep Casadesús
Feb 12, 2015·Helicobacter·Lorinda TurnerRichard L Ferrero
Feb 5, 2009·FEMS Microbiology Letters·Mónica N GiacomodonatoMaría Cristina Cerquetti
Jan 26, 2012·PLoS Genetics·Sara B HernándezJosep Casadesús
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Feb 21, 2006·Nature Reviews. Microbiology·Didier Wion, Josep Casadesús
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Aug 19, 2011·The Journal of Biological Chemistry·Francisco García-del PortilloM Graciela Pucciarelli
Jun 9, 2005·Microbiology·Victoria L TaylorPetra C F Oyston
Dec 22, 2005·Infection and Immunity·Tatiana E ErovaAshok K Chopra
Sep 4, 2008·Infection and Immunity·Douglas M HeithoffMichael J Mahan
Sep 23, 2008·Journal of Bacteriology·Marcello JakominJosep Casadesús
Sep 26, 2006·Journal of Bacteriology·Roberto BalbontínJosep Casadesús
Nov 18, 2005·Journal of Bacteriology·Ana AlonsoFrancisco García-del Portillo
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Nov 16, 2019·Nature Reviews. Microbiology·María A Sánchez-Romero, Josep Casadesús
Aug 5, 2006·Genetics·Ana I PrietoJosep Casadesús
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Jun 7, 2019·Molecular Carcinogenesis·Pallavi Kompella, Karen M Vasquez
Dec 22, 2004·Genetics·Ana I PrietoJosep Casadesús

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