Envelope protein palmitoylations are crucial for murine coronavirus assembly

Journal of Virology
Joseph A BoscarinoThomas M Gallagher

Abstract

The coronavirus assembly process encloses a ribonucleoprotein genome into vesicles containing the lipid-embedded proteins S (spike), E (envelope), and M (membrane). This process depends on interactions with membranes that may involve palmitoylation, a common posttranslational lipidation of cysteine residues. To determine whether specific palmitoylations influence coronavirus assembly, we introduced plasmid DNAs encoding mouse hepatitis coronavirus (MHV) S, E, M, and N (nucleocapsid) into 293T cells and found that virus-like particles (VLPs) were robustly assembled and secreted into culture medium. Palmitate adducts predicted on cysteines 40, 44, and 47 of the 83-residue E protein were then evaluated by constructing mutant cDNAs with alanine or glycine codon substitutions at one or more of these positions. Triple-substituted proteins (E.Ts) lacked palmitate adducts. Both native E and E.T proteins localized at identical perinuclear locations, and both copurified with M proteins, but E.T was entirely incompetent for VLP production. In the presence of the E.T proteins, the M protein subunits accumulated into detergent-insoluble complexes that failed to secrete from cells, while native E proteins mobilized M into detergent-soluble s...Continue Reading

References

Oct 1, 1991·The Journal of Cell Biology·A M Swift, C E Machamer
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·C YangR W Compans
Oct 1, 1995·The Journal of Cell Biology·D J OpsteltenP J Rottier
Sep 1, 1993·The Journal of Cell Biology·O A WeiszC E Machamer
May 9, 2000·Journal of Virology·C A de HaanP J Rottier
Mar 30, 2001·Virology·J MaedaS Makino
Jan 5, 2002·Journal of Virology·Emily Corse, Carolyn E Machamer
Jan 5, 2002·Journal of Virology·Kristopher M CurtisRalph S Baric
Mar 29, 2003·Journal of Virology·Lili Kuo, Paul S Masters
Sep 16, 2003·FEBS Letters·Maria Eugenia Gonzalez, Luis Carrasco
Oct 15, 2003·Journal of Virology·Cornelis A M de HaanPeter J M Rottier
Nov 6, 2004·Virology·Lauren WilsonGary Ewart
Dec 22, 2004·Current Topics in Microbiology and Immunology·P S Masters, P J M Rottier
Jun 21, 2005·FEBS Letters·Vanessa MadanLuis Carrasco
Sep 6, 2005·Advances in Virus Research·Cornelis A M de Haan, Peter J M Rottier
Nov 18, 2005·The Journal of Biological Chemistry·Yuichiro KidaMasao Sakaguchi
Aug 1, 2006·Advances in Virus Research·Paul S Masters
Aug 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Wei LuBing Sun
Sep 26, 2006·FEBS Letters·Manuel Bauer, Lucas Pelkmans
Oct 3, 2006·Methods : a Companion to Methods in Enzymology·Renaldo C DrisdelWilliam N Green
Oct 14, 2006·Advances in Experimental Medicine and Biology·Klara K ErikssonVolker Thiel

❮ Previous
Next ❯

Citations

Sep 29, 2009·The Journal of Biological Chemistry·Nathalie MajeauDenis Leclerc
Oct 6, 2009·The Journal of Biological Chemistry·Ana Shulla, Tom Gallagher
May 8, 2009·Journal of Virology·Kelley R HurstPaul S Masters
Aug 12, 2014·Viruses·Ruth McBrideBurtram C Fielding
Apr 10, 2015·Viruses·Makoto Ujike, Fumihiro Taguchi
Jul 24, 2012·Protein Expression and Purification·Krupakar ParthasarathyJaume Torres
Jan 11, 2016·Wiley Interdisciplinary Reviews. RNA·Binbin DingMingzhou Chen
Oct 7, 2015·Trends in Microbiology·Christopher M GoodwinJoshua Munger
May 3, 2012·Biology of the Cell·Michael Veit
Mar 3, 2015·Virology·Pavithra VenkatagopalanBrenda G Hogue
Dec 7, 2010·Journal of Structural Biology·Benjamin W NeumanMichael J Buchmeier
Aug 6, 2014·Virus Research·Marta L DeDiegoLuis Enjuanes
Sep 6, 2018·PloS One·Antonina NaskalskaKrzysztof Pyrc
May 28, 2019·Virology Journal·Dewald Schoeman, Burtram C Fielding
Dec 7, 2019·Expert Opinion on Drug Discovery·Mohamed Rasheed Gadalla, Michael Veit
Jul 31, 2020·Archives of Physiology and Biochemistry·Heena Rehman, Md Iftekhar Ahmad
May 17, 2012·Viruses·Travis R Ruch, Carolyn E Machamer
Jun 1, 2018·Future Virology·To Sing Fung, Ding Xiang Liu
Mar 24, 2020·Future Virology·Entedar A J Alsaadi, Ian M Jones
Nov 26, 2020·Genetics and Molecular Biology·Fernando Moreira SimabucoArmando Morais Ventura
Nov 24, 2020·Future Virology·Sk Manirul HaqueAbdul Kalam Azad John Mohamed
Jan 15, 2021·Viruses·Jerome E Tanner, Caroline Alfieri
Feb 3, 2021·International Journal of Molecular Sciences·Nicholas A Wong, Milton H Saier
Feb 9, 2021·Computational and Structural Biotechnology Journal·Yeu-Yang TsengWei-Li Hsu
May 22, 2021·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Thomas Sean LendvayMay C Chu
Jul 22, 2021·Biochemistry. Biokhimii︠a︡·Larisa V Kordyukova, Andrey V Shanko
Jul 29, 2021·Cell Reports·Caroline G WilliamsChristopher F Basler
Jul 23, 2021·Bioscience Reports·Rajesh AhirwarSaroj Kumar
Sep 14, 2021·Journal of Chemical Theory and Computation·Shengjie SunLin Li
Oct 3, 2021·Developmental Cell·Francisco S MesquitaF Gisou van der Goot
Nov 9, 2021·Frontiers in Microbiology·Antonina NaskalskaKrzysztof Pyrc

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.