Environment Polarity in Proteins Mapped Noninvasively by FTIR Spectroscopy.

The Journal of Physical Chemistry Letters
Joshua ManorIsaiah T Arkin

Abstract

The polarity pattern of a macromolecule is of utmost importance to its structure and function. For example, one of the main driving forces for protein folding is the burial of hydrophobic residues. Yet polarity remains a difficult property to measure experimentally, due in part to its non-uniformity in the protein interior. Herein, we show that FTIR linewidth analysis of noninvasive 1-(13)C=(18)O labels can be used to obtain a reliable measure of the local polarity, even in a highly multi-phasic system, such as a membrane protein. We show that in the Influenza M2 H(+) channel, residues that line the pore are located in an environment that is as polar as fully solvated residues, while residues that face the lipid acyl chains are located in an apolar environment. Taken together, FTIR linewidth analysis is a powerful, yet chemically non-perturbing approach to examine one of the most important properties in proteins - polarity.

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Citations

Mar 5, 2014·Proceedings of the National Academy of Sciences of the United States of America·Esther S Feldblum, Isaiah T Arkin
Aug 5, 2015·Journal of the American Chemical Society·Bei DingZhan Chen
Dec 1, 2012·Biochimica Et Biophysica Acta·Joshua Manor, Isaiah T Arkin
Dec 4, 2012·Biochimica Et Biophysica Acta·Yechiel Shai
Jan 1, 2014·Journal of the American Chemical Society·Jennifer E LaaserMartin T Zanni
Oct 21, 2016·Physical Chemistry Chemical Physics : PCCP·Beatrice N MarkiewiczFeng Gai
Aug 1, 2020·Journal of Computational Chemistry·Ioannis StylianakisAntonios Kolocouris
Dec 7, 2013·The Journal of Physical Chemistry. B·Stephen D FriedVijay S Pande
May 29, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Baixiong ZhangShuji Ye
Aug 19, 2015·The Journal of Physical Chemistry Letters·Joshua ManorIsaiah T Arkin
Aug 21, 2013·Biochemistry·Sean D MoranMartin T Zanni
Jun 30, 2018·The Journal of Physical Chemistry Letters·Esther S Brielle, Isaiah T Arkin

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