Environmental Controls of Oyster-Pathogenic Vibrio spp. in Oregon Estuaries and a Shellfish Hatchery

Applied and Environmental Microbiology
Mary R GradovilleAngelicque E White

Abstract

Vibrio spp. have been a persistent concern for coastal bivalve hatcheries, which are vulnerable to environmental pathogens in the seawater used for rearing larvae, yet the biogeochemical drivers of oyster-pathogenic Vibrio spp. in their planktonic state are poorly understood. Here, we present data tracking oyster-pathogenic Vibrio bacteria in Netarts Bay and Yaquina Bay in Oregon, USA, as well as in adjacent coastal waters and a local shellfish hatchery, through the 2015 upwelling season. Vibrio populations were quantified using a culture-independent approach of high-throughput Vibrio-specific 16S rRNA gene sequencing paired with droplet digital PCR, and abundances were analyzed in the context of local biogeochemistry. The most abundant putative pathogen in our samples was Vibrio coralliilyticus Environmental concentrations of total Vibrio spp. and V. coralliilyticus were highest in Netarts Bay sediment samples and higher in seawater from Netarts Bay than from nearshore coastal waters or Yaquina Bay. In Netarts Bay, the highest V. coralliilyticus concentrations were observed during low tide, and abundances increased throughout the summer. We hypothesize that the warm shallow waters in estuarine mudflats facilitate the local gro...Continue Reading

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Citations

Mar 17, 2019·Applied and Environmental Microbiology·Xiaolei WangXiao-Hua Zhang
Apr 26, 2020·Fish & Shellfish Immunology·Andrew N Robinson, Timothy J Green
Sep 20, 2019·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Lindsay AlmaJacqueline L Padilla-Gamiño
Apr 5, 2021·Environmental Science and Pollution Research International·Ibukun M AdesiyanAnthony I Okoh

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Methods Mentioned

BETA
PCR
flow cytometry
amplicon sequencing
PCRs

Software Mentioned

R package relaimpo
Fluidigm
QIIME
QuantaSoft
XTide
ANOSIM
PRIMER
MiSeq Standard
PhyML
R

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