Environmental effects on transglutaminase production and cell sporulation in submerged cultivation of Bacillus circulans

Applied Biochemistry and Biotechnology
Claucia Fernanda Volken de SouzaMarco Antônio Záchia Ayub

Abstract

In this research, the effects of pH, temperature, and oxygen on growth kinetics of a newly isolated strain of Bacillus circulans from the Amazon and their correlations with transglutaminase (TGase) production and cell sporulation were investigated. Statistical experimental methods were used to optimize these parameters, while induction of sporulation was achieved by oxygen culture control. Full factorial composite experimental design and response surface methodology were experimentally tested. The model showed that temperature has a positive and significant effect on TGase production (P < 0.05) while pH and temperature, associated with anoxic conditions, have a marked effect on cell sporulation which is consistently linked with TGase production. The contour plot of results showed that the best culture conditions for TGase production of B. circulans were 30 degrees C, initial pH 8.5, and the highest production was obtained in late-stationary culture phase with maximal specific enzyme activity of 655 U g(-1) of cells (0.37 U/mL). A correlation between enzyme production and cell sporulation, as mediated by oxygen culture conditions, was also demonstrated and, although demonstrated only for B. subtilis, it corroborates the molecula...Continue Reading

References

Dec 27, 2002·The Journal of General and Applied Microbiology·Katsunori KobayashiShigeru Yamanaka
Jan 17, 2003·Biotechnology and Applied Biochemistry·Luís Henrique de Barros SoaresMarco Antônio Záchia Ayub
Aug 20, 2004·Journal of Bacteriology·Katerina Ragkousi, Peter Setlow
Nov 4, 2005·Journal of Bacteriology·Rita ZilhãoAdriano O Henriques

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Citations

Jan 17, 2019·Preparative Biochemistry & Biotechnology·Karuna L Sorde, Laxmi Ananthanarayan
Oct 8, 2020·International Journal of Environmental Research and Public Health·Xuan ChenHui Wei

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