Environmental endocrine disruptors promote adipogenesis in the 3T3-L1 cell line through glucocorticoid receptor activation.

Obesity
Robert M SargisMatthew J Brady

Abstract

The burgeoning obesity and diabetes epidemics threaten health worldwide, yet the molecular mechanisms underlying these phenomena are incompletely understood. Recently, attention has focused on the potential contributions of environmental pollutants that act as endocrine disrupting chemicals (EDCs) in the pathogenesis of metabolic diseases. Because glucocorticoid signaling is central to adipocyte differentiation, the ability of EDCs to stimulate the glucocorticoid receptor (GR) and drive adipogenesis was assessed in the 3T3-L1 cell line. Various EDCs were screened for glucocorticoid-like activity using a luciferase reporter construct, and four (bisphenol A (BPA), dicyclohexyl phthalate (DCHP), endrin, and tolylfluanid (TF)) were shown to significantly stimulate GR without significant activation of the peroxisome proliferator-activated receptor-gamma. 3T3-L1 preadipocytes were then treated with EDCs and a weak differentiation cocktail containing dehydrocorticosterone (DHC) in place of the synthetic dexamethasone. The capacity of these compounds to promote adipogenesis was assessed by quantitative oil red O staining and immunoblotting for adipocyte-specific proteins. The four EDCs increased lipid accumulation in the differentiatin...Continue Reading

References

Dec 1, 1988·The Journal of Clinical Endocrinology and Metabolism·M Rebuffé-ScriveP Björntorp
Jul 23, 1998·Physiological Reviews·F M GregoireH S Sul
Nov 30, 1998·Environmental Health Perspectives·M JohanssonB O Lund
Jun 4, 1999·Seminars in Cell & Developmental Biology·R M CowherdR E McGehee
Apr 25, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·L C HodgesC L Walker
Dec 18, 2001·Experimental Biology and Medicine·F M Gregoire
Jan 19, 2002·Biochemical and Biophysical Research Communications·Mathias FasshauerRalf Paschke
May 15, 2002·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Paula F Baillie-Hamilton
Jun 14, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Christopher H Hurst, David J Waxman
Aug 7, 2003·Biochemical and Biophysical Research Communications·Atanas G AtanasovAlex Odermatt
Jul 21, 2004·Environmental Research·Begoña BotellaNicolás Olea
Jan 22, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hiroshi MasunoKatsuhisa Honda
Aug 5, 2005·The Journal of Endocrinology·Nicole Draper, Paul M Stewart
Nov 3, 2005·Environmental Health Perspectives·Atanas G AtanasovAlex Odermatt
Apr 15, 2006·Molecular Endocrinology·Felix GrünBruce Blumberg
Sep 20, 2006·International Journal of Clinical Practice·J Wilding
Oct 19, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Alex OdermattAnna A Dzyakanchuk
Feb 22, 2007·The Journal of Biological Chemistry·Jaime KimMatthew J Brady
Jan 17, 2008·Environmental Health Perspectives·Antonia M CalafatLarry L Needham
Jun 10, 2008·The Journal of Clinical Endocrinology and Metabolism·Daniel H Bessesen
Jun 26, 2008·Reproductive Toxicology·Young Joo LeeHae-Seong Yoon
Nov 21, 2008·Nutrition Reviews·Lu Qi, Young Ae Cho
May 1, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Peter Josef HofmannJosef Köhrle

❮ Previous
Next ❯

Citations

Oct 24, 2012·International Journal of Obesity : Journal of the International Association for the Study of Obesity·J WangX Li
Jul 28, 2011·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Stéphanie Decherf, Barbara A Demeneix
Sep 8, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Pan HuLing Zhao
Oct 23, 2012·Environmental Health : a Global Access Science Source·He-xing WangQing-wu Jiang
Mar 9, 2010·Italian Journal of Pediatrics·Giuseppe LatiniLorenzo Iughetti
Sep 12, 2013·Critical Care : the Official Journal of the Critical Care Forum·Mirna MarquesLies Langouche
Mar 16, 2012·Endocrine Reviews·Laura N VandenbergJohn Peterson Myers
Oct 7, 2010·Environmental Health Perspectives·Michelle A MendezJordi Sunyer
May 17, 2012·Environmental Health Perspectives·Juliane-Susanne SchmidtBernd Fischer
Nov 2, 2011·Environmental Health Perspectives·Suzanne M Snedeker, Anthony G Hay
Apr 12, 2013·Oxidative Medicine and Cellular Longevity·Carla LubranoLucio Gnessi
Nov 9, 2010·Annual Review of Physiology·Cristina Casals-Casas, Béatrice Desvergne
Jul 12, 2014·International Journal of Environmental Research and Public Health·Marijke de CockMargot van de Bor
Dec 10, 2015·Environmental Science & Technology·Jianyun ZhangWeiping Liu
Jun 26, 2013·Annales d'endocrinologie·Patrick FenichelFrançoise Brucker-Davis
Sep 27, 2012·Neurotoxicology and Teratology·Jared J SchwartzerRobert F Berman
Jun 11, 2011·Aquatic Toxicology·C RibeccoO Carnevali
Jan 11, 2011·International Journal of Hygiene and Environmental Health·Nathalie ArnichJacques Tulliez
Jul 30, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Jana VitkuRichard Hampl
May 29, 2015·Environmental Health Perspectives·Jonathan G BoucherElla Atlas
Jul 26, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Hsin-Chieh LanJyun-Hong Lin
Jan 25, 2011·The Mount Sinai Journal of Medicine, New York·Michele La Merrill, Linda S Birnbaum
Mar 23, 2011·Birth Defects Research. Part C, Embryo Today : Reviews·Amanda Janesick, Bruce Blumberg
Sep 21, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Alice MarmugiLaïla Mselli-Lakhal
Mar 22, 2016·Biochemical and Biophysical Research Communications·Xiuwen XieGang Li
Jun 6, 2013·Biochimica Et Biophysica Acta·Shane M Regnier, Robert M Sargis
Nov 15, 2014·Critical Reviews in Toxicology·Okhyun LeeCharles R Tyler
May 15, 2015·Environmental Health : a Global Access Science Source·Fabien LagardeChristophe Rousselle
Feb 23, 2013·Molecular and Cellular Endocrinology·Anna Pereira-FernandesRonny Blust
Sep 7, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Katra KolšekMarija Sollner Dolenc
May 25, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Beverly S Rubin
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·L Le CorreM-C Chagnon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.