Environmental Exposure of Sperm Sex-Chromosomes: A Gender Selection Technique

Toxicological Research
Ibukun P OyeyipoStefan S du Plessis

Abstract

Preconceptual sex selection is still a highly debatable process whereby X- and Y-chromosome-bearing spermatozoa are isolated prior to fertilization of the oocyte. Although various separation techniques are available, none can guarantee 100% accuracy. The aim of this study was to separate X- and Y-chromosome-bearing spermatozoa using methods based on the viability difference between the X- and Y-chromosome-bearing spermatozoa. A total of 18 experimental semen samples were used, written consent was obtained from all donors and results were analysed in a blinded fashion. Spermatozoa were exposed to different pH values (5.5, 6.5, 7.5, 8.5, and 9.5), increased temperatures (37°C, 41°C, and 45°C) and ROS level (50 μM, 750 μM, and 1,000 μM). The live and dead cell separation was done through a modified swim-up technique. Changes in the sex-chromosome ratio of samples were established by double-label fluorescent in situ hybridization (FISH) before and after processing. The results indicated successful enrichment of Xchromosome-bearing spermatozoa upon incubation in acidic media, increased temperatures, and elevated H2O2. This study demonstrated the potential role for exploring the physiological differences between X-and Y-chromosome-be...Continue Reading

References

Nov 11, 1993·Nature·K H Cui, C D Matthews
Dec 1, 1996·Human Reproduction·K Dawson, A Trounson
Dec 1, 1996·Molecular Human Reproduction·S P Flaherty, C D Matthews
Apr 27, 2017·Toxicological Research·Hye Lyun JeonSoo Jung Sohn

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Citations

Oct 24, 2019·Veterinary World·Nidhi P RavalChaitanya G Joshi
Feb 11, 2020·Frontiers in Cell and Developmental Biology·Md Saidur Rahman, Myung-Geol Pang
Aug 20, 2020·Reproduction in Domestic Animals = Zuchthygiene·Maryam MoradiBijan Soleymani
Jul 13, 2021·Systems Biology in Reproductive Medicine·Rajasingam S JeyendranMorry B Fiddler
Oct 19, 2021·Frontiers in Cell and Developmental Biology·Qifu HeFusheng Quan

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