Environmental factors influence P. aeruginosa binding to the wounded mouse cornea

Current Eye Research
L ChenL D Hazlett

Abstract

To determine whether environmental factors or bacterial viability affect the binding of two strains of P. aeruginosa to mouse cornea. Scarified corneas were placed in organ culture and inoculated with P. aeruginosa cell suspensions containing either ATCC 19660 or PAO1 bacterial strains classed as cytotoxic or invasive, respectively. Eyes were incubated in vitro for 1 h after bacterial application at different pH or temperature conditions or in PBS containing various divalent cations. The adhesion of heat-killed or formalin-fixed bacteria was tested similarly. Scanning electron microscopy (scanning EM) was used to quantitate adherent bacteria. P. aeruginosa ATCC 19660 showed an increase in binding at pH 8.0, favored higher temperatures and required both calcium and magnesium for optimum binding. Adherence of PAO1 was enhanced at pH 6.5 and decreased at pH 8.0. This strain favored binding at lower temperatures and did not require either divalent cation for optimum binding. In addition, the presence of magnesium ions resulted in reduced binding for this strain. Both strains exhibited less binding ability after formalin fixation or heat killing. Environmental factors and bacterial viability are important factors which influence the...Continue Reading

References

Feb 1, 1992·Molecular Microbiology·V J DiRita
Jan 1, 1989·International Review of Cytology·R J Doyle, E M Sonnenfeld
Aug 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L D Hazlett, P Mathieu
Mar 1, 1985·The British Journal of Ophthalmology·M S InslerL A Wilson
Nov 1, 1972·Archives of Ophthalmology·P R Laibson
Jan 1, 1982·Current Eye Research·R W ReichertZ S Zam
Apr 1, 1995·Infection and Immunity·S Favre-BonteC Forestier
Aug 1, 1993·Infection and Immunity·S C StraleyJ B Bliska
Jun 1, 1996·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·K SachseH Pfützner
Dec 1, 1996·Journal of Medical Microbiology·J P ThornleyA Eley

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Citations

Aug 9, 2002·DNA and Cell Biology·Michael E ZegansGeorge O'Toole
Dec 25, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Qingjun ZhouLixin Xie
Jul 12, 2001·Clinical & Experimental Ophthalmology·L P Aristoteli, M D Willcox
Oct 23, 2004·Eye & Contact Lens·H Kathleen Dannelly, Rosaline V Waworuntu
Jun 15, 2016·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Joseph M CollacoGarry R Cutting

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