Environmental factors shaping the community structure of ammonia-oxidizing bacteria and archaea in sugarcane field soil

Microbes and Environments
Kanako TagoMasahito Hayatsu

Abstract

The effects of environmental factors such as pH and nutrient content on the ecology of ammonia-oxidizing bacteria (AOB) and archaea (AOA) in soil has been extensively studied using experimental fields. However, how these environmental factors intricately influence the community structure of AOB and AOA in soil from farmers' fields is unclear. In the present study, the abundance and diversity of AOB and AOA in soils collected from farmers' sugarcane fields were investigated using quantitative PCR and barcoded pyrosequencing targeting the ammonia monooxygenase alpha subunit (amoA) gene. The abundances of AOB and AOA amoA genes were estimated to be in the range of 1.8 × 10(5)-9.2 × 10(6) and 1.7 × 10(6)-5.3 × 10(7) gene copies g dry soil(-1), respectively. The abundance of both AOB and AOA positively correlated with the potential nitrification rate. The dominant sequence reads of AOB and AOA were placed in Nitrosospira-related and Nitrososphaera-related clusters in all soils, respectively, which varied at the level of their sub-clusters in each soil. The relationship between these ammonia-oxidizing community structures and soil pH was shown to be significant by the Mantel test. The relative abundances of the OTU1 of Nitrosospira c...Continue Reading

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Citations

Jun 29, 2016·Environmental Science and Pollution Research International·Ademir Sérgio Ferreira AraújoRadomir Schmidt
May 22, 2021·Ecotoxicology and Environmental Safety·Bingjun HanKeqiang Zhang

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Datasets Mentioned

BETA
AB569307
ATCC25196
U91603
DRA002432

Methods Mentioned

BETA
PCR
Assay

Software Mentioned

Mothur
MEGA5
SPSS
Mafft

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