Environmental history impacts gene expression during diapause development in the alfalfa leafcutting bee, Megachile rotundata

The Journal of Experimental Biology
George D YocumJ H Bowsher

Abstract

Our understanding of the mechanisms controlling insect diapause has increased dramatically with the introduction of global gene expression techniques, such as RNA sequencing (RNA-seq). However, little attention has been given to how ecologically relevant field conditions may affect gene expression during diapause development because previous studies have focused on laboratory-reared and -maintained insects. To determine whether gene expression differs between laboratory and field conditions, prepupae of the alfalfa leafcutting bee, Megachile rotundata, entering diapause early or late in the growing season were collected. These two groups were further subdivided in early autumn into laboratory- and field-maintained groups, resulting in four experimental treatments of diapausing prepupae: early and late field, and early and late laboratory. RNA-seq and differential expression analyses were performed on bees from the four treatment groups in November, January, March and May. The number of treatment-specific differentially expressed genes (97 to 1249) outnumbered the number of differentially regulated genes common to all four treatments (14 to 229), indicating that exposure to laboratory or field conditions had a major impact on ge...Continue Reading

References

Apr 1, 1995·Genes & Development·M P BelvinK V Anderson
May 5, 1995·The Journal of Biological Chemistry·P RayA Ray
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Apr 20, 2001·Cytokine & Growth Factor Reviews·D JoyceR G Pestell
Dec 1, 2001·Annual Review of Entomology·David L Denlinger
Oct 31, 2002·Cancer Letters·Howard DoongElise C Kohn
Nov 5, 2004·Cellular and Molecular Life Sciences : CMLS·E I CamposG Li
Dec 8, 2005·Journal of Insect Physiology·Vladimír Kostál
Aug 10, 2006·Nature Reviews. Molecular Cell Biology·Jan-Michael Peters
Oct 20, 2006·Journal of Insect Physiology·G D YocumJ N Knoblett
Feb 12, 2008·Cellular and Molecular Life Sciences : CMLS·M Kabbage, M B Dickman
Sep 10, 2008·Journal of Cellular Physiology·Andrew H Coles, Stephen N Jones
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Feb 25, 2009·Nature Reviews. Cancer·Marcos Malumbres, Mariano Barbacid
Dec 17, 2009·BMC Bioinformatics·Christiam CamachoThomas L Madden
Feb 6, 2010·Genome Biology·Matthew D YoungAlicia Oshlack
May 15, 2010·Cell Division·Jorrit M Enserink, Richard D Kolodner
Jul 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gregory J RaglandDaniel A Hahn
Aug 31, 2010·Cellular and Molecular Life Sciences : CMLS·Almass-Houd Aguissa-TouréGang Li
Sep 3, 2010·Annual Review of Entomology·Theresa L Pitts-Singer, James H Cane
Sep 30, 2010·BMC Bioinformatics·Murray P CoxPatrick J Biggs
Jan 19, 2012·Environmental Entomology·Kevin M O'NeillCasey M Delphia
May 24, 2012·The EMBO Journal·Christof Niehrs, Sergio P Acebron
Mar 22, 2013·Proceedings. Biological Sciences·Monica F PoelchauPeter A Armbruster
Jun 17, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Diana L Huestis, Tovi Lehmann
Apr 22, 2015·PLoS Neglected Tropical Diseases·Xin HuangPeter A Armbruster
Jun 11, 2015·Bioinformatics·Felipe A SimãoEvgeny M Zdobnov

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Citations

Jun 8, 2019·Integrative and Comparative Biology·Dacotah MelicherJulia H Bowsher
Sep 21, 2019·Current Opinion in Insect Science·Gregory J RaglandMegan E Meuti
Mar 20, 2021·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Lizzette D CambronKendra J Greenlee
Oct 14, 2021·Molecular Ecology·Sean T BresnahanChristina M Grozinger

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