PMID: 11934498Apr 6, 2002Paper

Environmental investigation of potentially pathogenic Vibrio parahaemolyticus in the Seto-Inland Sea, Japan

FEMS Microbiology Letters
Muhammad Jahangir AlamSumio Shinoda

Abstract

Seawater and organic material (live and/or dead matter deposited on any substratum submersed in seawater) were collected during the cool weather season from a coast of the Seto-Inland Sea, Japan, and analyzed to determine Vibrio parahaemolyticus densities and the occurrence of pathogenic strains, defined as those possessing tdh and/or trh genes by the polymerase chain reaction (PCR), using isolated DNA from enrichment culture of the samples. About 95% of the samples were positive for V. parahaemolyticus (with densities of 3 to >1400 cells per 100 ml water or 10 g organic samples) by the most-probable-number (MPN)-PCR technique with species-specific toxR primers, but only 40% were positive by the conventional MPN-culture technique (with densities ranging from 3 to 240 cells per 100 ml water or 10 g organics). Furthermore, the tdh and trh genes were positive in 55% and 20% of samples, respectively, by the MPN-PCR technique. No tdh and trh gene-positive strains were isolated by the conventional MPN-culture procedure. The difference in detection between the MPN-culture and the MPN-PCR techniques appeared to be significant and may be attributed to different detection sensitivities and other factors.

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Citations

May 11, 2013·Molecular and Cellular Probes·Muhammad Tofazzal HossainIn-Soo Kong
Mar 25, 2004·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Norinaga MiwaMasato Akiyama
Jun 14, 2014·Molecular and Cellular Probes·Peiyan HeWenjie Gao
Oct 7, 2004·Applied and Environmental Microbiology·Jorge Frias-LopezBruce W Fouke
Nov 6, 2004·Applied and Environmental Microbiology·María Eugenia Cabrera-GarcíaElsa Irma Quiñones-Ramírez
Aug 18, 2009·Applied and Environmental Microbiology·Munirul AlamRita R Colwell
Oct 30, 2012·Applied and Environmental Microbiology·Gregory DickinsonSunny C Jiang
Oct 12, 2005·FEMS Microbiology Letters·Lesley Maurice BilungMitsuaki Nishibuchi
Jul 10, 2008·Current Microbiology·Xiaoyan LuanXiao-Hua Zhang
Jan 6, 2009·Journal of Applied Microbiology·A B EllingsenL M Rørvik
Dec 13, 2012·World Journal of Microbiology & Biotechnology·Piyanuch PrompamornParin Chaivisuthangkura
Oct 1, 2010·Environmental Microbiology Reports·Akiko HamamotoAkira Takahashi
Oct 12, 2013·Journal of Basic Microbiology·Min XuHaijin Mou
Jan 8, 2015·Frontiers in Cellular and Infection Microbiology·Shimaa S El-MalahXinan Jiao
Jul 23, 2003·Environmental Microbiology·M Jahangir AlamSumio Shinoda
Mar 24, 2015·Frontiers in Microbiology·Rongzhi WangShihua Wang
Jan 24, 2018·Journal of Food Protection·Rundong WangRavi Gooneratne
Feb 12, 2009·Journal of Food Protection·Hidemasa KodakaHideaki Matsuoka
Dec 24, 2015·Frontiers in Microbiology·Vengadesh LetchumananKok-Gan Chan
Feb 18, 2015·Frontiers in Microbiology·Vengadesh LetchumananKok-Gan Chan
Oct 7, 2016·Environmental Monitoring and Assessment·Guadalupe Barrera-EscorciaMarcial Leonardo Lizárraga-Partida
Oct 11, 2017·International Journal of Environmental Research and Public Health·Charles A Osunla, Anthony I Okoh
May 10, 2017·Frontiers in Microbiology·Vengadesh LetchumananLearn-Han Lee
Nov 19, 2017·Applied and Environmental Microbiology·Sara ElmahdiJohn Jacobs
Aug 15, 2021·Environmental Microbiology·Kyle D BrumfieldRita R Colwell

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