PMID: 9538504Apr 16, 1998Paper

Environmental KCl causes an upregulation of apical membrane maxi K and ENaC channels in everted Ambystoma collecting tubule

The Journal of Membrane Biology
L C Stoner, S C Viggiano

Abstract

Patch clamp methods were used to characterize the channels on the apical membrane of initial collecting ducts from Ambystoma tigrinum. Apical membranes were exposed by everting and perfusing fragments of the renal tubule in vitro. Tubules were dissected from two groups of animals; one maintained in tap water, and the other kept in a solution of 50 mM KCl from seven to nineteen days. Patches of apical membranes on tubules taken from animals exposed to tap water expressed low-conductance amiloride sensitive sodium channels (ENaC) in 22 of 49 patches. Only three maxi K channels were observed in this group. In animals exposed to KCl, low-conductance amiloride sensitive sodium channels, 3.7 +/- 0.2 pS (36 of 45 patches) and high-conductance 98.3 +/- 5.0 pS (19 of 45 patches) potassium channels were observed. The estimated density of apical maxi K channels increased dramatically from 0.08 to 0.76 channels/mu 2 in tubules taken from animals exposed to KCl. All but four of nineteen patches which contained maxi K channels also expressed the low conductance sodium channels. Therefore, at least 85% of the maxi K channels studied were in principal cells. We speculate that the increase in maxi K channel activity may represent a mechanism fo...Continue Reading

Citations

May 5, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Nadja MøbjergIvana Novak
Apr 12, 2000·Kidney International·L G Palmer, G Frindt
Aug 19, 2007·Current Opinion in Nephrology and Hypertension·P Richard Grimm, Steven C Sansom
Jun 27, 2008·American Journal of Physiology. Renal Physiology·Genevieve EstiloLisa M Satlin
Dec 25, 2004·Physiological Reviews·Steven C HebertWenhui Wang
Apr 12, 2014·Current Opinion in Pharmacology·Donghai WenSteven C Sansom
May 26, 2005·American Journal of Physiology. Renal Physiology·Fadi NajjarLisa M Satlin
Jul 20, 2006·American Journal of Physiology. Renal Physiology·Lisa M SatlinThomas R Kleyman
Oct 26, 2006·American Journal of Physiology. Renal Physiology·Lawrence G Palmer, Gustavo Frindt
Sep 13, 2002·American Journal of Physiology. Cell Physiology·N Yvonne Tallini, Larry C Stoner
Dec 4, 2015·American Journal of Physiology. Cell Physiology·Rolando Carrisoza-GaytanLisa M Satlin
Dec 15, 2015·American Journal of Physiology. Renal Physiology·Tennille N WebbMarcelo D Carattino
Dec 22, 1999·The American Journal of Physiology·L G Palmer
Feb 10, 1999·The American Journal of Physiology·L C Stoner, S C Viggiano
Nov 8, 2002·Biochimica Et Biophysica Acta·Niels J WillumsenE Hviid Larsen
Jul 8, 2021·American Journal of Physiology. Renal Physiology·Evan C RayLisa M Satlin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.