PMID: 39627Jul 4, 1979

Environmental pH modulation of collagenase in normal human fibroblast cultures

Biochimica Et Biophysica Acta
D F Busiek, E A Bauer


Normal human skin fibroblast cultures have been used to assess the effects of relatively minor changes in environmental pH on collagenase, a major extracellular gene product. Collagenase accumulation in the culture medium, measured both as enzyme activity and immunoreactive material, was 2- to 10-fold greater at pH 7.6--8.2 than at pH 6.8--7.2. The pH-associated increase in collagenase was paralleled by an increase in general protein synthesis. Nevertheless, prototypic lysosomal and cytoplasmic enzymes changed very little under identical culturing conditions. Although substantial intracellular protein degradation occurred at all pH values, the small differences either in general protein degradation or in specific collagenase degradation in the medium were of insufficient magnitude to account for the increased accumulation of collagenase.


Dec 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J ArnonG Bach
Mar 1, 1982·Collagen and Related Research·L RyhänenJ Uitto
Oct 1, 1984·Collagen and Related Research·H G WelgusG P Stricklin
Apr 1, 1982·Journal of the American Academy of Dermatology·E A BauerA Z Eisen
Feb 1, 1985·Scandinavian Journal of Dental Research·K Helgeland
Jan 1, 1988·Annals of the New York Academy of Sciences·P A Riley


Mar 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·J M DayerD R Robinson
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E A Bauer
Apr 1, 1977·Journal of Dental Research·G G Rose, P B Robertson
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S O LieE F Neufeld
Oct 1, 1966·Biochemistry·Y NagaiJ Gross
Oct 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·F WROBLEWSKI, J S LADUE

Related Concepts

Protein Degradation, Regulatory
Enzymes, antithrombotic
Skin Fibroblast
Protein Degradation, Metabolic
Protein Biosynthesis
Enzyme Activity
Enzymes for Treatment of Wounds and Ulcers

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