Environmental sampling for detection of Mycobacterium avium ssp. paratuberculosis on large California dairies

Journal of Dairy Science
Roy D BerghausIan A Gardner

Abstract

Environmental samples collected from each of 3 locations on 23 large California dairies were cultured to evaluate the utility of this approach for identifying herds infected with Mycobacterium avium ssp. paratuberculosis. Results were compared with concurrent ELISA testing of > or = 60 animals in each herd, and with previously performed individual and pooled fecal cultures of 60 animals. The estimated proportions of infected herds did not differ significantly among the testing methods (environmental sampling, 74%; previous fecal culture, 70%; and concurrent ELISA testing, 65%). Measures of agreement between environmental sampling and the results of previous fecal cultures were 70% (observed agreement), 85% (positive agreement), 62% (negative agreement), and 0.47 (kappa), whereas agreement between environmental sampling and concurrent ELISA testing was 65, 75, and 43%, and 0.19, for the same measures, respectively. The proportion of positive environmental samples on each farm was significantly correlated with the proportion of seropositive animals (r = 0.53), suggesting that environmental sampling may also provide a qualitative estimate of within-herd prevalence. Of the sampling locations that were evaluated, samples of lagoon w...Continue Reading

References

Jan 1, 1990·Journal of Clinical Epidemiology·D V Cicchetti, A R Feinstein
Jan 1, 1990·Journal of Clinical Epidemiology·A R Feinstein, D V Cicchetti
Jan 1, 1988·Journal of Clinical Epidemiology·W D Thompson, S D Walter
Sep 1, 1997·Infection and Immunity·J D CirilloL E Bermudez
Nov 5, 1997·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J R Stabel
Jul 13, 2002·Preventive Veterinary Medicine·Huybert GroenendaalJan Willem Hesselink
May 9, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·G van SchaikS J Shin
Aug 6, 2003·Preventive Veterinary Medicine·John M Adaska, Randall J Anderson
Jul 1, 1956·Anesthesiology·R W VIRTUE, B E JONES
Jul 9, 2004·Journal of the American Veterinary Medical Association·Heather L HirstGregory M Goodell
Feb 11, 2005·Journal of the American Veterinary Medical Association·Steven H HendrickMarie Archambault
Jun 9, 2005·Clinical and Diagnostic Laboratory Immunology·Michael T CollinsRobert H Whitlock

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Citations

Jul 27, 2010·Applied and Environmental Microbiology·Susanne W F EisenbergMirjam Nielen
Feb 22, 2012·The Veterinary Quarterly·Susanne W F EisenbergAd P Koets
Mar 29, 2014·Critical Reviews in Microbiology·Geoffrey N ElliottColin D Campbell
Aug 28, 2007·Preventive Veterinary Medicine·Saraya TavornpanichIan A Gardner
Jul 19, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Sharif S AlyIan A Gardner
Dec 19, 2006·Journal of the American Veterinary Medical Association·Michael T CollinsScott J Wells
Jun 15, 2019·BMC Veterinary Research·Richard WhittingtonJacobus H de Waard
Oct 15, 2018·Journal of Dairy Science·Caroline S CorbettHerman W Barkema
Jul 26, 2011·Veterinary Medicine International·J A Fernández-SilvaM Bülte

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