Environmental Screening of Fonsecaea Agents of Chromoblastomycosis Using Rolling Circle Amplification

Journal of Fungi
Morgana F VoidaleskiV A Vicente

Abstract

Chromoblastomycosis is a chronic, cutaneous or subcutaneous mycosis characterized by the presence of muriform cells in host tissue. Implantation disease is caused by melanized fungi related to black yeasts, which, in humid tropical climates, are mainly members of the genus Fonsecaea. In endemic areas of Brazil, F. pedrosoi and F. monophora are the prevalent species. The current hypothesis of infection is traumatic introduction via plant materials, especially by plant thorns. However, isolation studies have demonstrated a low frequency of the agents in environmental substrates. The present study aimed to detect F. pedrosoi and F. monophora in shells of babassu coconuts, soil, plant debris, and thorns from endemic areas of chromoblastomycosis in Maranhão state, northern Brazil, using Rolling Circle Amplification (RCA) with padlock probes as a new environmental screening tool for agents of chromoblastomycosis. In addition to molecular screening, the environmental samples were analyzed by fungal isolation using mineral oil flotation. The limit of detection of the RCA method was 2.88 × 107 copies of DNA per sample for the used padlock probes, indicating that this represents an efficient and sensitive molecular tool for the environme...Continue Reading

References

Jul 1, 1996·The American Journal of Tropical Medicine and Hygiene·P EsterreJ L Pecarrere
Apr 2, 2004·Revista Do Instituto De Medicina Tropical De São Paulo·Claudio Guedes SalgadoUbirajara Imbiriba Salgado
Nov 7, 2006·Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology·Sirlei Garcia MarquesJackson Maurício Lopes Costa
Jan 1, 2008·Studies in Mycology·V A VicenteA Pizzirani-Kleiner
Feb 14, 2012·Journal of Microbiological Methods·Mahdi DavariG Sybren de Hoog
Jul 6, 2012·Medical Mycology·Thales Domingos ArantesEduardo Bagagli
Mar 30, 2013·Mycopathologia·Raquel Schier GuerraWalter A Boeger
Sep 14, 2013·Mycopathologia·Treepradab NorkaewYoshitsugu Miyazaki
Jul 2, 2014·Clinical Microbiology Reviews·Seyedmojtaba SeyedmousaviG Sybren de Hoog
Oct 16, 2015·Revista Do Instituto De Medicina Tropical De São Paulo·Flavio Queiroz-Telles
Dec 24, 2015·Frontiers in Microbiology·Anderson M RodriguesZoilo P de Camargo
Jan 20, 2016·Fungal Biology·Laszlo IrinyiWieland Meyer
Nov 20, 2016·Clinical Microbiology Reviews·Flavio Queiroz-TellesThomas J Walsh
Mar 30, 2017·Studies in Mycology·M M TeixeiraG Sybren de Hoog
May 2, 2017·Medical Mycology Case Reports·Daiane HeidrichMaria Lucia Scroferneker
Aug 5, 2017·PLoS Neglected Tropical Diseases·Reshu AgarwalImmaculata Xess
Oct 25, 2017·Frontiers in Microbiology·Vania A VicenteSybren De Hoog
Oct 26, 2018·Frontiers in Microbiology·Gheniffer FornariSybren de Hoog
Mar 30, 2020·Fungal Biology·Bruna Jacomel Favoreto de Souza LimaVânia Aparecida Vicente

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Methods Mentioned

BETA
Rolling Circle Amplification
PCR

Software Mentioned

Mega

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