Environmental signals influence expression of a cortical areal phenotype in vitro independent of effects on progenitor cell proliferation

Developmental Biology
R T FerriP Levitt

Abstract

We have shown previously that, in vitro, cortical progenitor cells isolated from specific locations of the cerebral wall can adopt area-specific fates, assayed by expression of the limbic system-associated membrane protein (LAMP; R. T. Ferri and P. Levitt, Cereb. Cortex 3, 187-198, 1993). Progenitors destined to produce LAMP neurons, however, will differentiate to express the limbic molecular phenotype if grown with TGFalpha and collagen type IV (R. T. Ferri and P. Levitt, Development 121, 1151-1160, 1995), while other signals fail to induce LAMP. The present study used BrdU labeling of progenitor cells to examine whether modulation of LAMP expression was paralleled by predictable changes in cell proliferation. The general pattern of proliferation is similar under a variety of culture conditions: approximately half the cells are mitotic, and activity is always highest during the first 24 hr in vitro, with little cell division occurring by the third day. Moreover, the rate of proliferation, in the presence or absence of TGFalpha, is the same on all substrates tested, with the exception of laminin. The TGFalpha/collagen type IV signaling system that induces LAMP expression by the precursors has no modulating effect on their proli...Continue Reading

Citations

Jun 28, 2000·Progress in Neurobiology·M P Junier
Mar 19, 2002·Progress in Neurobiology·Lukas Sommer, Mahendra Rao
Apr 20, 2011·Biologie aujourd'hui·Marie-Pierre Junier, Ariane Sharif
Jan 1, 1997·Annual Review of Neuroscience·P LevittK L Eagleson
Jun 30, 2006·Journal of Neurochemistry·Diane M Jaworski, Leonor Pérez-Martínez
Sep 8, 2004·Brain Research. Developmental Brain Research·Hsiao T NiPhillip K Peterson

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