Environmental variables influencing in vitro development of hamster 2-cell embryos to the blastocyst stage

Biology of Reproduction
S H McKiernan, B D Bavister

Abstract

This study is a systematic analysis of environmental variables influencing development of 2-cell hamster embryos to the blastocyst stage in vitro. Experiments were done using a chemically defined (protein-free) culture medium (HECM-2). Physicochemical variables examined were temperature, the concentrations of CO2, HCO3-, Ca2+, Mg2+, K+, and O2, the presence of a silicone oil overlay, and osmotic pressure. The optimal temperature for development in vitro was 37.5 degrees C; lower temperatures were inhibitory. There was no significant effect on blastocyst development of alterations in the concentrations of NaHCO3, CaCl2, MgCl2, and KCl, or in the ratios of Ca2+:Mg2+ and Na+:K+, over the ranges tested. Development to the blastocyst stage was significantly stimulated by increased CO2 concentrations (7.5% and 10%), by reduced O2 concentrations (10% and 5%), and by the presence of silicone oil overlying the culture medium. Reduction of blastocyst development in the absence of an oil overlay was not caused by increased osmotic pressure. Cleavage stage embryos were not affected by osmolalities ranging from 250 to 350 mOsmols, but blastocyst development was inhibited at greater than or equal to 325 mOsmols. Under optimized conditions (3...Continue Reading

Citations

Jan 1, 1996·Molecular Reproduction and Development·D K Barnett, B D Bavister
Jul 21, 1998·Molecular Reproduction and Development·M LaneB D Bavister
Jul 28, 1999·Molecular Reproduction and Development·M TakahashiA Okano
Dec 1, 1995·Molecular Reproduction and Development·J J Eppig, K Wigglesworth
Nov 1, 1991·Cytotechnology·J H ResauS J Meltzer
Dec 29, 2000·Theriogenology·H ZhouB D Bavister
Mar 29, 2000·Theriogenology·B D Bavister
Dec 4, 2001·Journal of Reproductive Immunology·P B SeshagiriR P Rao
Jan 1, 1997·Free Radical Biology & Medicine·T YamashitaM Kuwabara
Dec 25, 2007·Reproduction, Fertility, and Development·Michelle LaneDeirdre L Zander-Fox
Apr 1, 1999·Human Reproduction·J C DumoulinJ L Evers
Mar 5, 2004·Biology of Reproduction·Kosuke HaigoToshitaka Horiuchi
Nov 3, 2011·Reproductive Biology and Endocrinology : RB&E·David B Gomes SobrinhoJosé G Franco
Mar 28, 2012·The Journal of Reproduction and Development·Masashi Takahashi
Mar 16, 2006·The Journal of Reproduction and Development·Toshitaka Horiuchi
Mar 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·S H McKiernan, B D Bavister
Sep 1, 1994·In Vitro Cellular & Developmental Biology. Animal·K KobayashiH Hoshi
May 3, 2011·Reproductive Biomedicine Online·Tanya V SabhnaniJoyce C Harper
May 18, 2007·Reproduction in Domestic Animals = Zuchthygiene·G G LeoniS Naitana
Oct 30, 2004·Theriogenology·Ni Wayan Kurniani KarjaTakeshige Otoi
Jan 14, 2005·The Journal of Reproduction and Development·Hirotada TsujiiKoh-ichi Hamano
Aug 1, 2007·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Z G WangZ R Xu

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.