Enzymatic activity of CD26 (dipeptidylpeptidase IV) is not required for its signalling function in T cells

Immunobiology
M HegenB Fleischer

Abstract

CD26 is a proteolytic enzyme (dipeptidylpeptidase IV) expressed on the T cell surface that defines an alternative activation signal for human T lymphocytes. Crosslinking of CD26 via monoclonal antibodies triggers proliferation and cytotoxicity in preactivated T cells. In this study, we used highly specific competitive and irreversible inhibitors of dipeptidylpeptidase IV to study the role of the enzymatic activity in activation of CD26-transfected T cells as well as of CD26-expressing normal human T cell clones. These inhibitors at concentrations that blocked up to 95% of the enzymatic activity, did not specifically inhibit T cell activation neither via TCR/CD3 nor via CD26 itself. This demonstrates that the enzymatic activity of CD26 is not required for its T cell activating properties.

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Citations

Apr 1, 1994·Immunology Today·B Fleischer
Jan 5, 2002·Biochimica Et Biophysica Acta·A Sedo, R Malík
Feb 1, 1997·Cellular Signalling·R Resta, L F Thompson
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Feb 14, 2003·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Georges VauquelinPatrick Vanderheyden
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Feb 7, 2015·American Journal of Physiology. Renal Physiology·Yoshiki HigashijimaMasaomi Nangaku
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Jan 1, 1997·Journal of Enzyme Inhibition·C A PargellisT P Pitner
Mar 22, 2003·European Journal of Cell Biology·Emil Boonacker, Cornelis J F Van Noorden

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