Enzymatic and immunochemical evaluation of phospholipid hydroperoxide glutathione peroxidase (PHGPx) in testes and epididymal spermatozoa of rats of different ages

International Journal of Andrology
Federica TramerEnrico Panfili

Abstract

Selenium (Se) and selenoproteins such as glutathione peroxidases are necessary for the proper development and fertilizing capacity of sperm cells. Phospholipid hydroperoxide glutathione peroxidase (PHGPx, E.C. 1.11.1.12) is a monomeric seleno-enzyme present in different mammalian tissues in soluble and bound form. Its function, like the other glutathione peroxidases, was originally viewed as a protective role against hydroperoxides, but direct and indirect evidence indicates that it has additional regulatory roles. PHGPx is present in testis cells and sperm cells, and its appearance is hormone regulated. We present here biochemical data, which clearly indicate that the enzyme specific activity in rat is age-dependent during the life-span monitored (from 36 to 365 days), with a maximum at 3 months of age in the testis germ cells and at 6 months of age in the isolated epididymal sperm cells. Western blotting and immunocytochemical analysis by means of anti-PHGPx antibodies show the different distribution and the strong binding of PHGPx in the testes and sperm cell subcellular compartments (nucleus, acrosome, mitochondria and residual bodies) of rats of different age. The presence of the protein exhibits in the testis cells a patt...Continue Reading

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Citations

Dec 21, 2006·Journal of Molecular Histology·Kelly WindmillGreg R Collier
Dec 21, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Xiaoli ChenDong Wang
Sep 27, 2005·Contraception·Rossella PuglisiCarla Boitani
Jul 10, 2013·Experimental Gerontology·Tiago Boeira SalomonMara Silveira Benfato
May 6, 2014·Frontiers in Endocrinology·Giulia GuerrieroGaetano Ciarcia
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Mar 20, 2019·Proteomics·Giovanni C Forcina, Scott J Dixon
Dec 20, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Pandian NagakannanEftekhar Eftekharpour
Nov 1, 2020·Biomolecules·Riccardo TurchiKatia Aquilano

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