PMID: 6163468Mar 13, 1981Paper

Enzymatic and immunological properties of the protease form of aminopeptidase N and A from pig and rabbit intestinal brush border

Biochimica Et Biophysica Acta
H FeracciS Maroux

Abstract

Immunological homology was shown between the active site regions of pig and rabbit aminopeptidases N and between those of the corresponding aminopeptidases A. However, no homology was detectable between the aminopeptidases N and A (EC 3.4.11.-) in a given species. The dimeric structure of pig aminopeptidases did not significantly modify their catalytic properties in aqueous solution compared to those of the monomeric rabbit enzymes. Only a slight difference in binding conditions was noted in the case of aminopeptidases N. Aminopeptidase A activity towards acidic substrates was enhanced by physiological concentrations of Ca2+ while that towards neutral substrates was considerably reduced. Therefore, acidic amino acid residues in proteins and peptides may be assumed to be mostly split off in vivo by aminopeptidase A, neutral residues by aminopeptidases N and basic residues by both enzymes. The respective specificity of aminopeptidase A and N for acidic and neutral amino acid residues was found to be mainly due to a more productive binding mode of the substrate rather than to a better affinity.

References

Mar 1, 1979·The Biochemical Journal·C Doumeng, S Maroux
Jul 15, 1975·Biochemistry·C W Garner, F J Behal
Apr 8, 1976·Biochimica Et Biophysica Acta·H WackerE A Stein
Jul 30, 1974·Biochemistry·C W Garner, F J Behal
Sep 15, 1973·Biochimica Et Biophysica Acta·S MarouxJ Baratti
Jun 1, 1980·European Journal of Biochemistry·A Benajiba, S Maroux
Jul 1, 1980·Biochimica Et Biophysica Acta·H Feracci, S Maroux
Sep 9, 1980·Biochimica Et Biophysica Acta·J P GorvelS Maroux

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Citations

Apr 1, 1988·Journal of Bioenergetics and Biomembranes·S P Shirazi-BeecheyR B Beechey
Jan 1, 1985·The Journal of Membrane Biology·H FeracciS Maroux
Mar 1, 1994·Comparative Biochemistry and Physiology. Biochemistry and Molecular Biology·I Skrtić, L Vitale
Dec 1, 1995·Journal of Gastroenterology·H Terashima, N W Bunnett
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D M NanusA P Albino
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J Wang, M D Cooper
Jul 1, 1985·Journal of Pharmaceutical Sciences·R E Stratford, V H Lee
Apr 16, 2008·Journal of Neurochemistry·Nadia de MotaCatherine Llorens-Cortes
Aug 31, 2012·The Journal of Biological Chemistry·Alan H M WongJames M Rini
Jun 23, 2006·The Journal of Biological Chemistry·Yoshikuni GotoMasafumi Tsujimoto
Sep 1, 1994·Biological Chemistry Hoppe-Seyler·S MentzelK J Assmann
Jul 26, 1982·Biochimica Et Biophysica Acta·D GratecosM Sémériva
Sep 1, 1988·Biochimie·S MarouxD Louvard

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