Enzymatic biodiesel synthesis from yeast oil using immobilized recombinant Rhizopus oryzae lipase

Bioresource Technology
Susan Hartwig DuarteFrancisco Valero

Abstract

The recombinant Rhizopus oryzae lipase (1-3 positional selective), immobilized on Relizyme OD403, has been applied to the production of biodiesel using single cell oil from Candida sp. LEB-M3 growing on glycerol from biodiesel process. The composition of microbial oil is quite similar in terms of saponifiable lipids than olive oil, although with a higher amount of saturated fatty acids. The reaction was carried out in a solvent system, and n-hexane showed the best performance in terms of yield and easy recovery. The strategy selected for acyl acceptor addition was a stepwise methanol addition using crude and neutralized single cell oil, olive oil and oleic acid as substrates. A FAMEs yield of 40.6% was obtained with microbial oils lower than olive oil 54.3%. Finally in terms of stability, only a lost about 30% after 6 reutilizations were achieved.

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Citations

Sep 24, 2015·Bioresource Technology·Yun WangGao-Ying Zhi
Jan 21, 2017·Journal of Industrial Microbiology & Biotechnology·Renata GudiukaiteDonaldas Citavicius
Sep 24, 2019·Biotechnology and Bioengineering·Mercedes LlamasElia Tomás-Pejó
Nov 25, 2017·Current Opinion in Biotechnology·Shinji HamaAkihiko Kondo
Dec 19, 2018·Journal of Agricultural and Food Chemistry·Xiao-Feng LiXinglong Xiao
Jul 18, 2017·New Biotechnology·Albert CanetPatrick Adlercreutz

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