PMID: 7028481Sep 1, 1981Paper

Enzymatic catalysis in the affinity labelling of liver alcohol dehydrogenase with haloacids

European Journal of Biochemistry
K H Dahl, J S McKinley-McKee

Abstract

In this work, the inactivation at pH 7.0 of liver alcohol dehydrogenase by iodoacetamide and a series of six haloacids has been studied, and the kinetic constants determined. Enzyme inactivation was compared with the model alkylation of a metal-thiol and a thiolate anion free in solution. The following conclusions resulted. 1. Inactivation of liver alcohol dehydrogenase by iodoacetamide is a direct thiol alkylation, while inactivation by selective alkylation of Cys-46 by the haloacids is facilitated by reversible complex formation. 2. Inactivation half-time for the haloacids ranged over 4-190 min, a difference mainly caused by dissimilar chemical reactivities rather than diverse fitting in the active site. 3. The thiol of Cys-46 is alkylated as a zinc-thiol complex. It is, as such, not especially reactive; indeed it has a nucleophilic reactivity similar to that observed with the model compound free in solution. 4. Affinity labelling of liver alcohol dehydrogenase by haloacids compared with alkylation of the similar group free in solution illustrates enzymatic catalysis by reversible complex formation. With the present series of 'substrates' a rate enhancement of up to 58 000 is seen.

References

Mar 25, 1976·Journal of Molecular Biology·H EklundA Akeson
May 1, 1970·European Journal of Biochemistry·C H ReynoldsJ S McKinley-McKee
Oct 1, 1969·European Journal of Biochemistry·C H Reynolds, J S McKinley-McKee
May 1, 1968·European Journal of Biochemistry·N Evans, B R Rabin
Feb 1, 1966·Biochemistry·N E GoodR M Singh
Jan 1, 1980·European Journal of Biochemistry·K H Dahl, J S McKinley-McKee
Dec 1, 1981·European Journal of Biochemistry·K H Dahl, J S McKinley-McKee
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN

❮ Previous
Next ❯

Citations

May 1, 1997·Protein Science : a Publication of the Protein Society·M PeretzY Burstein
Dec 1, 1981·European Journal of Biochemistry·K H Dahl, J S McKinley-McKee
Jul 1, 1982·Bioscience Reports·L SkjeldalJ S McKinley-McKee
Apr 24, 2021·Inorganic Chemistry·Xuemei YangMarcetta Y Darensbourg
Apr 18, 2001·Chemico-biological Interactions·Y PockerC C Bhat

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.