Enzymatic characterization of peroxisomal and cytosolic betaine aldehyde dehydrogenases in barley

Physiologia Plantarum
Takashi FujiwaraTetsuko Takabe

Abstract

Betaine aldehyde dehydrogenase (BADH; EC 1.2.1.8) is an important enzyme that catalyzes the last step in the synthesis of glycine betaine, a compatible solute accumulated by many plants under various abiotic stresses. In barley (Hordeum vulgare L.), we reported previously the existence of two BADH genes (BBD1 and BBD2) and their corresponding proteins, peroxisomal BADH (BBD1) and cytosolic BADH (BBD2). To investigate their enzymatic properties, we expressed them in Escherichia coli and purified both proteins. Enzymatic analysis indicated that the affinity of BBD2 for betaine aldehyde was reasonable as other plant BADHs, but BBD1 showed extremely low affinity for betaine aldehyde with apparent K(m) of 18.9 microM and 19.9 mM, respectively. In addition, V(max)/K(m) with betaine aldehyde of BBD2 was about 2000-fold higher than that of BBD1, suggesting that BBD2 plays a main role in glycine betaine synthesis in barley plants. However, BBD1 catalyzed the oxidation of omega-aminoaldehydes such as 4-aminobutyraldehyde and 3-aminopropionaldehyde as efficiently as BBD2. We also found that both BBDs oxidized 4-N-trimethylaminobutyraldehyde and 3-N-trimethylaminopropionaldehyde.

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Citations

Mar 15, 2012·Histochemistry and Cell Biology·Markus IslingerMichael Schrader
Oct 1, 2011·The Protein Journal·Ratree WongpanyaKiattawee Choowongkomon
Feb 15, 2013·The Journal of Biological Chemistry·David KopečnySolange Moréra
Oct 24, 2009·FEBS Letters·Shiro MitsuyaTetsuko Takabe
Dec 12, 2012·Chemico-biological Interactions·Héctor Riveros-RosasRosario A Muñoz-Clares
Feb 21, 2009·Plant Biology·T L FitzgeraldR J Henry
Oct 16, 2010·Plant, Cell & Environment·Tony H H Chen, Norio Murata
Mar 23, 2019·Frontiers in Plant Science·Maria Grazia AnnunziataPetronia Carillo
Feb 1, 2011·Functional Plant Biology : FPB·Petronia CarilloRonan Sulpice

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