Enzymatic characterization of recombinant mouse retinal dehydrogenase type 1

Biochemical Pharmacology
Isabelle GagnonPangala V Bhat

Abstract

Retinal dehydrogenases (RALDHs) convert retinal into retinoic acids (RAs), which are important signaling molecules in embryogenesis and tissue differentiation. We expressed mouse RALDH type 1 (mRALDH1) in Escherichia coli and studied the kinetic properties of the recombinant enzyme for retinal substrates. Purified recombinant mRALDH1 catalyzed the oxidation of all-trans and 9-cis retinal but not 13-cis retinal, and exhibited two pH optimums, 7.8 and 9.4, for all-trans and 9-cis retinal substrates, respectively. The K(m) for all-trans retinal (11.6 micro M) was 3-fold higher than for 9-cis retinal (3.59 micro M). However, the conversion efficiencies of either all-trans or 9-cis retinal to the respective RAs were similar. MgCl(2) inhibited the oxidation of both all-trans and 9-cis retinal. Chloral hydrate and acetaldehyde competitively suppressed all-trans retinal oxidation with inhibition constants (K(i)) of 4.99 and 49.4 micro M, respectively. Retinol, on the other hand, blocked the reaction uncompetitively. These data extend the kinetic characterization of mRALDH1, provide insight into the possible role of this enzyme in the biogenesis of RAs, and should give useful information on the determination of amino acid residues that ...Continue Reading

References

Nov 17, 1988·Biochimica Et Biophysica Acta·P V BhatA Lacroix
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·P V BhatX L Wang
Apr 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H L Ang, G Duester
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Mar 8, 2002·The Journal of Biological Chemistry·Veronique MontplaisirSylvie Mader
May 2, 2002·Biochimica Et Biophysica Acta·Isabelle GagnonPangala V Bhat

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Citations

Feb 5, 2010·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Qian ShenWei Guo
May 25, 2005·The Journal of Biological Chemistry·Alexander R MoiseKrzysztof Palczewski
Dec 15, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Hélène BrodeurPangala V Bhat
Nov 15, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Aurelia SimaPangala Bhat
Mar 26, 2011·Molecular Endocrinology·Barbara ReichertOuliana Ziouzenkova
Apr 11, 2006·Zoological Science·Farhana Babli Rahman, Kiyoshi Yamauchi
May 1, 2013·Journal of Lipid Research·Natalia Y Kedishvili
Dec 13, 2006·Médecine sciences : M/S·Maxime ParisottoSylvie Mader
Jul 10, 2008·Expert Opinion on Drug Metabolism & Toxicology·Satori A MarchittiVasilis Vasiliou
Sep 22, 2009·Biochimica Et Biophysica Acta·Aurelia SimaPangala V Bhat
Oct 6, 2007·Biochimica Et Biophysica Acta·Hélène BrodeurPangala V Bhat
Oct 26, 2005·The Biochemical Journal·Caroline E GrahamMartin J Warren
Oct 13, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jean-Francois LandrierRalph Rühl
Dec 14, 2004·Toxicology and Applied Pharmacology·Géraldine LemaireRoger Rahmani

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