PMID: 8959740Jan 1, 1996Paper

Enzymatic cleavage of thymopoietin oligopeptides by pancreatic and intestinal brush-border enzymes

Peptides
J HeizmannS Wolffram

Abstract

The intestinal enzymatic degradation of the immunomodulating peptides thymotrinan (TP3), thymocartin (TP4), and thymopentin (TP5), three oligopeptides derived from the naturally occurring thymus hormone thymopoietin, was investigated to evaluate their potential for peroral drug delivery. In the presence of brush-border membrane vesicles, crude pancreas extract and everted rings from duodenum, jejunum, ileum, and colon, all peptides were shown to be degraded both by pancreatic enzymes and brush-border aminopeptidases. Degradation clearances (Cldeg) of TP3, TP4, and TP5 were calculated for a quantitative comparison of peptide stability. In the presence of crude pancreas extract, there was a rapid degradation of TP5 (Cldeg 17.9 ml/min) in comparison with TP3 and TP4 (Cldeg 0.95 and 0.56 ml/min, respectively, at 0.2 mM peptide concentration) caused by the cleavage of the C-terminal tyrosine by carboxypeptidase A, whereas TP3 and TP4 underwent hydrolysis by aminopeptidase N. In the presence of brush-border membrane vesicles, the degradation clearances were 3.9, 3.1, and 2.4 ml/min at 0.2 mM concentrations of TP4, TP5, and TP3, respectively. The clearance of all peptides was lowered with increasing peptide concentrations, indicating ...Continue Reading

References

May 1, 1990·The Journal of Allergy and Clinical Immunology·D Y LeungJ M Hanifin
Jan 1, 1990·Cancer Immunology, Immunotherapy : CII·L DénesK Lapis
Apr 1, 1989·British Journal of Rheumatology·B K KanthariaS P Liyanage
Jan 1, 1986·International Journal of Immunopharmacology·E RajnavölgyiJ Gergely
Jan 1, 1994·The Journal of Pharmacy and Pharmacology·P LangguthH P Merkle
Feb 1, 1993·The Annals of Thoracic Surgery·A MarkewitzB Reichart
Jan 1, 1993·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·A MarkewitzB Reichart

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Citations

Aug 1, 1998·Journal of Controlled Release : Official Journal of the Controlled Release Society·A Bernkop-Schnürch
Mar 7, 2012·Journal of Chemical Information and Modeling·Karim M ElSawyLeo S D Caves
Jan 10, 2002·Journal of Pharmaceutical Sciences·Pascale AnderleHans P Merkle
Sep 10, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Qingfang MengYuanguo Cheng
May 14, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mao-Bo ChengQiang Zhang
Sep 16, 2003·Biopharmaceutics & Drug Disposition·D WerdenbergP Langguth
Aug 10, 2000·The Journal of Pharmacy and Pharmacology·E KrondahlH Lennernäs

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