PMID: 7524089Oct 11, 1994Paper

Enzymatic completion of mammalian lagging-strand DNA replication

Proceedings of the National Academy of Sciences of the United States of America
J J TurchiRobert A Bambara

Abstract

Using purified proteins from calf and a synthetic substrate, we have reconstituted the enzymatic reactions required for mammalian Okazaki fragment processing in vitro. The required reactions are removal of initiator RNA, synthesis from an upstream fragment to generate a nick, and then ligation. With our substrate, RNase H type I (RNase HI) makes a single cut in the initiator RNA, one nucleotide 5' of the RNA-DNA junction. The double strand specific 5' to 3' exonuclease removes the remaining monoribonucleotide. After dissociation of cleaved RNA, synthesis by DNA polymerase generates a nick, which is then sealed by DNA ligase I. The unique specificities of the two nucleases for primers with initiator RNA strongly suggest that they perform the same reactions in vivo.

References

Mar 15, 1977·European Journal of Biochemistry·W BüsenP Hausen
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G SiegalR A Bambara
Jan 17, 1991·Biochimica Et Biophysica Acta·R A Bambara, C B Jessee
Feb 1, 1991·Analytical Biochemistry·M Goulian, C J Heard
Sep 21, 1990·Cell·A MorrisonA Sugino
Jan 1, 1989·Annual Review of Cell Biology·B Stillman
Nov 1, 1985·Cell·L M JohnsonJ L Campbell

❮ Previous
Next ❯

Citations

Jan 1, 1997·Journal of Molecular Evolution·T Shirai, M Go
May 19, 1998·Leukemia Research·L A Henricksen, R A Bambara
Oct 31, 2003·Critical Reviews in Oncology/hematology·Waleed Abdel-AzizRobert J Hickey
Oct 20, 2005·Journal of Bioscience and Bioengineering·N OhtaniS Kanaya
Sep 4, 2012·The Journal of Biological Chemistry·Zhenxin HuStephen J Benkovic
Feb 3, 2009·The Journal of Biological Chemistry·Young-Hoon KangYeon-Soo Seo
Jul 18, 2012·The Journal of Experimental Medicine·Bjoern HillerAxel Roers
Dec 17, 1997·Molecular Biology of the Cell·D F Fiorentino, G R Crabtree
Feb 28, 1998·Human Molecular Genetics·J K Schweitzer, D M Livingston
Feb 1, 2011·Journal of Molecular Cell Biology·Li Zheng, Binghui Shen
Aug 1, 1998·Nucleic Acids Research·J L Alleva, P W Doetsch
Aug 16, 2001·Nucleic Acids Research·P Wilkins StevensD M Kelso
Feb 14, 2002·Nucleic Acids Research·Byung-in Lee BiDavid M Wilson
Aug 11, 2004·Nucleic Acids Research·Gi-Hyuck RyuYeon-Soo Seo
Nov 11, 2010·Nucleic Acids Research·Dorjbal DorjsurenAnton Simeonov
Jan 20, 2011·Nucleic Acids Research·Doryen BubeckAndrew P Jackson
Apr 15, 2004·Molecular and Cellular Biology·Yuan LiuCatherine H Freudenreich
Aug 2, 2005·Molecular and Cellular Biology·Koji KikuchiShunichi Takeda
Oct 6, 1998·Annual Review of Biochemistry·S Waga, B Stillman
Jun 11, 2004·Annual Review of Biochemistry·Yuan LiuRobert A Bambara
May 12, 2005·Molecular Cancer Research : MCR·Heather J BoeckmanJohn J Turchi
Jul 4, 2013·Nucleic Acids Research·Fei Sun, Li Huang
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·P FrankW Büsen
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·R S MuranteR A Bambara

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.