Enzymatic conversion of all-trans-beta-carotene to retinal.

Methods in Enzymology
M R Lakshman, C Okoh

Abstract

Enzymatic conversion of all-trans-beta-carotene to retinal by a partially purified enzyme from rabbit, rat, and human neonatal intestinal mucosa has been demonstrated. The enzymatic product was characterized based on the following evidence. First, the product gave rise to its O-ethyl oxime by treatment with O-ethylhydroxylamine with an absorption maximum at 363 nm in ethanol characteristic of authentic retinal (O-ethyl) oxime. High-performance liquid chromatography of this derivative yielded a sharp peak with a retention time of 7.99 min, corresponding to the authentic compound. The enzyme blank and boiled enzyme blank failed to show any significant HPLC peaks corresponding to retinal (O-ethyl) oxime or retinal or retinol. Second, the mass spectrum of the O-ethyl oxime of the enzymatic product was identical to that of authentic retinal (O-ethyl) oxime (m/z 327, 45%; m+ and m/z 282, 100%, methoxy). Third, the 14C radioactivity persisted to constant specific activity even after repeated crystallization of the retinal (O-ethyl) oxime isolated from the enzyme reaction with purified beta-[14C]carotene. Fourth, the enzymatic product exhibited an absorption maximum at 370 nm in light petroleum characteristic of authentic retinal. Furt...Continue Reading

Citations

Nov 28, 2000·The Journal of Biological Chemistry·T M RedmondF X Cunningham
Aug 5, 2000·The Journal of Nutrition·A B Barua, J A Olson
Dec 13, 2006·Pediatric Dermatology·Sivaramakrishnan Venkatesh KarthikDavid Isherwood
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·C RosatiG Giuliano
May 1, 2009·Biochemistry·Prakash BhosalePaul S Bernstein
Jun 8, 2001·Progress in Retinal and Eye Research·J K McBeeD R Pepperberg
Mar 15, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Monte S Willis, Frank H Wians
Jul 6, 2010·Archives of Biochemistry and Biophysics·Georg LietzGerald Rimbach

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