Enzymatic defenses against the toxicity of oxygen and of streptonigrin in Escherichia coli.

Journal of Bacteriology
H M Hassan, I Fridovich

Abstract

Anaerobically grown Escherichia coli K-12 contain only one superoxide dismutase and that is the iron-containing isozyme found in the periplasmic space. Exposure to oxygen caused the induction of a manganese-containing superoxide dismutase and of another, previously undescribed, superoxide dismutase, as well as of catalase and peroxidase. These inductions differed in their responsiveness towards oxygen. Thus the very low levels of oxygen present in deep, static, aerobic cultures were enough for nearly maximal induction of the manganese-superoxide dismutase. In contrast, induction of the new superoxide dismutase, catalase, and peroxidase required the much higher levels of oxygen achieved in vigorously agitated aerobic cultures. Anaerobically grown cells showed a much greater oxygen enhancement of the lethality of streptonigrin than did aerobically grown cells, in accord with the proposal that streptonigrin can serve as an intracellular source of superoxide. Anaerobically grown cells in which enzyme inductions were prevented by puromycin were damaged by exposure to air. This damage was evidenced both as a decline in viable cell count and as structural abnormalities evident under an electron microscope.

References

Jan 1, 1975·Annual Review of Biochemistry·I Fridovich
May 1, 1973·Journal of Bacteriology·E M Gregory, I Fridovich
Jun 1, 1973·Journal of Bacteriology·E M Gregory, I Fridovich
Sep 1, 1973·Journal of Bacteriology·E M GregoryI Fridovich
Feb 1, 1974·Journal of Bacteriology·E M GregoryI Fridovich
Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Jul 25, 1958·The Journal of Biophysical and Biochemical Cytology·M L WATSON

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Citations

Jan 1, 1992·Environmental and Molecular Mutagenesis·D A Pagano, E Zeiger
Mar 1, 1991·American Journal of Respiratory Cell and Molecular Biology·Y S HoJ D Crapo
Feb 1, 1997·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·B M Babior
Feb 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bernard M BabiorPaul Wentworth
Jul 22, 2010·The Journal of General and Applied Microbiology·Zehong SuAkira Takahashi
Apr 19, 2011·Applied and Environmental Microbiology·Andrew J DonatiWoo-Suk Chang
Apr 1, 1996·Redox Report : Communications in Free Radical Research·M D Scott, J W Eaton
Jan 1, 1998·Bioscience, Biotechnology, and Biochemistry·Y T KimS K Kim
Oct 1, 1978·Photochemistry and Photobiology·I Fridovich
Jan 1, 1986·Journal of Basic Microbiology·G S Allgood, J J Perry
Jan 1, 1989·Archives of Microbiology·K A Short, R P Blakemore
Jan 1, 1984·Critical Reviews in Toxicology·J DiGuiseppi, I Fridovich
Jan 1, 1987·CRC Critical Reviews in Biochemistry·J V BannisterG Rotilio
Aug 31, 1987·FEBS Letters·M E SchininàA M Michelson
Dec 1, 1980·FEBS Letters·J V BannisterW H Bannister
Oct 1, 1985·Journal of Applied Toxicology : JAT·C I WeiH P Misra
Jan 1, 1983·Veterinary Clinical Pathology·H S Bender, W R Chickering
Dec 1, 1995·The Journal of Biological Chemistry·M K ChaI H Kim
Apr 1, 1983·Journal of Bacteriology·M W Smith, F C Neidhardt
Mar 1, 1983·Journal of Bacteriology·M J BarberE J Rauckman
Oct 1, 1988·Journal of Bacteriology·G J MacMichael

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