Enzymatic degradation behavior and cytocompatibility of silk fibroin-starch-chitosan conjugate membranes

Materials Science & Engineering. C, Materials for Biological Applications
Erkan T BaranRui L Reis

Abstract

The objective of this study was to investigate the influence of silk fibroin and oxidized starch conjugation on the enzymatic degradation behavior and the cytocompatability of chitosan based biomaterials. The tensile stress of conjugate membranes, which was at 50 Megapascal (MPa) for the lowest fibroin and starch composition (10 weight percent (wt.%)), was decreased significantly with the increased content of fibroin and starch. The weight loss of conjugates in α-amylase was more notable when the starch concentration was the highest at 30 wt.%. The conjugates were resistant to the degradation by protease and lysozyme except for the conjugates with the lowest starch concentration. After 10 days of cell culture, the proliferation of osteoblast-like cells (SaOS-2) was stimulated significantly by higher fibroin compositions and the DNA synthesis on the conjugate with the highest fibroin (30 wt.%) was about two times more compared to the native chitosan. The light microscopy and the image analysis results showed that the cell area and the lengths were decreased significantly with higher fibroin/chitosan ratio. The study proved that the conjugation of fibroin and starch with the chitosan based biomaterials by the use of non-toxic red...Continue Reading

References

Mar 26, 2002·International Journal of Biological Macromolecules·Mingzhong LiShigenori Kuga
Nov 9, 2002·Biomaterials·Gregory H AltmanDavid L Kaplan
Jun 23, 2004·Biomaterials·Lorenz MeinelDavid L Kaplan
Dec 29, 2004·Biomaterials·Rebecca L HoranGregory H Altman
Jul 14, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Xianyan WangDavid L Kaplan
Jul 24, 2007·Biomaterials·Lorenz UebersaxLorenz Meinel
Oct 6, 2009·Advanced Drug Delivery Reviews·T Kean, M Thanou

❮ Previous
Next ❯

Citations

Feb 16, 2016·Carbohydrate Polymers·Oana Maria DragostinLenuta Profire
Dec 24, 2015·International Journal of Molecular Sciences·Oana Maria DragostinLenuta Profire
Apr 5, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Hazal AydogduAysen Tezcaner
Oct 7, 2016·Cytotechnology·Ya-Ching LinSheng-Chi Wu
Nov 4, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Z Z DingD L Kaplan
Apr 16, 2020·International Journal of Molecular Sciences·Muhammad Asim AkhtarAldo R Boccaccini

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.