Enzymatic dehairing of goat skins using alkaline protease from Bacillus sp. SB12

Protein Expression and Purification
Selmen BrikiAhmed Landoulsi

Abstract

The present paper reports the production, purification and biochemical characterization of an extracellular alkaline protease from Bacillus sp. SB12. The enzyme has been used as an alternative to conventional chemicals treatment for dehairing of goat skins. The protease was optimally active at 37 °C and pH 9. Starch at 2% (w/v) was used as the best carbon source and the addition of yeast extract and peptone at 1% each supported the maximum level of protease production in the presence of 5 mM Ca(2+). Protease purification was performed with ammonium sulphate precipitation at 70% saturated fraction followed by dialysis and gel filtration chromatography using Sephadex G-100. The purified enzyme was homogeneous on non-denaturing PAGE and appeared as a single band with an apparent molecular weight of 41 kDa. This enzyme was moderately thermostable and has a wide pH stability range extending from pH 7 to 11. It showed high tolerance toward surfactants agents and organic solvents while it was completely inhibited by PMSF indicating the serine protease type. Purified protease was used to remove hair from goat skin proving its potential application in leather processing industry. The results revealed that the protease has enhanced the q...Continue Reading

References

Jan 11, 2005·Applied and Environmental Microbiology·Alexandre J MacedoCarlos Termignoni
Apr 22, 2005·Journal of Industrial Microbiology & Biotechnology·B Ghorbel-FrikhaM Nasri
Oct 20, 2005·Journal of Bioscience and Bioengineering·H Ogino, H Ishikawa
Jun 20, 2006·Bioresource Technology·S S NilegaonkarS S Sarnaik

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Citations

Aug 10, 2017·Critical Reviews in Biotechnology·Fabiano Jares ContesiniHélia Harumi Sato
Aug 28, 2020·Frontiers in Microbiology·Iuliia Danilova, Margarita Sharipova
Oct 29, 2020·Preparative Biochemistry & Biotechnology·Anam KhalidZaigui Wang

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