Enzymatic deposition of gold nanoparticles at vertically aligned carbon nanotubes for electrochemical stripping analysis and ultrasensitive immunosensing of carcinoembryonic antigen

The Analyst
Liling DengAimin Yu

Abstract

Herein we combine the sandwich immunoreaction at a vertically aligned single-walled carbon nanotube (SWCNT)-based immunosensor and the enzymatically catalytic deposition of gold nanoparticles (Au NPs) by a gold nanoprobe to develop a novel electrochemical immunosensing method. The vertically arranged nanostructure was prepared through the covalent linking of terminally carboxylated SWCNTs at an aryldiazonium-modified electrode. It not only provides an excellent platform for the high density immobilization of antibodies to obtain the immunosensor but also serves as useful molecular wires to accelerate electron transfer during the electrochemical immunosensing process. Meanwhile, the enzymatic reaction of the nanoprobe prepared by surface functionalization of the nanocarrier of Au NPs by high-content glucoamylases can catalyze the deposition of a large number of Au NPs at the immunosensor. The electrochemical stripping analysis of these nanoparticles enabled the convenient signal transduction of the method. Due to the sensitive gold stripping analysis at the vertically aligned SWCNTs and the multi-enzyme signal amplification of the nanoprobe, the electrochemical signal response was greatly enhanced. Thus, the method can be used f...Continue Reading

References

Aug 24, 2006·Journal of the American Chemical Society·Xin YuJames F Rusling
Jul 29, 2011·Chemical Society Reviews·Maëlle PerfézouArben Merkoçi
Jul 28, 2012·ACS Nano·Bhaskara V ChikkaveeraiahXiaoyuan Chen
Dec 19, 2012·Analytica Chimica Acta·Xiaomei PeiDianping Tang
Jan 24, 2013·Journal of the American Chemical Society·Ling YanChaoyong James Yang
Apr 2, 2013·Biosensors & Bioelectronics·Ying WanChunhai Fan
Jul 13, 2013·Biosensors & Bioelectronics·Rashida AkterMd Aminur Rahman
May 20, 2015·Chemical Society Reviews·Frederico R BaptistaS J Quinn
Jul 19, 2016·Chemical Reviews·Mahmoud LabibShana O Kelley
Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal
Dec 17, 2017·Biosensors & Bioelectronics·Xuefang GuHeinz-Bernhard Kraatz

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Methods Mentioned

BETA
AFM
sandwich immunoassay
sandwich

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