PMID: 8448837Feb 1, 1993Paper

Enzymatic determination of triglyceride, free cholesterol, and total cholesterol in tissue lipid extracts

Clinical Biochemistry
T P CarrL L Rudel

Abstract

Triglyceride, free cholesterol, and total cholesterol were quantified in lipid extracts of liver and thoracic aorta from nonhuman primates using commercially available enzymatic reagents. Lipids were solubilized in water by the addition of Triton X-100. Results of the enzymatic assays compared favorably with chemical assays of lipids separated by thin-layer chromatography. In addition to saving time, the present method has the advantage of measuring each lipid class from a single sample preparation. Furthermore, the procedure has been adapted for use with microtiter plates that conserve both sample and reagent.

References

Sep 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·S E Carlson, S Goldfarb
Nov 1, 1984·Analytical Biochemistry·F Omodeo SalèB Berra

❮ Previous
Next ❯

Citations

May 1, 2009·European Journal of Nutrition·Heather E RasmussenJi-Young Lee
Sep 17, 2009·Molecules and Cells·Yanjun LuJun Tian
Feb 15, 2011·Digestive Diseases and Sciences·Nathan J ShoresLarry Rudel
Jun 1, 1995·Clinical Biochemistry·G B Janssen, G W Meijer
Mar 3, 2005·The Journal of Nutritional Biochemistry·Susan Hazels Mitmesser, Timothy P Carr
Nov 26, 2002·European Journal of Pharmacology·Toshiyuki FunatsuKeiji Miyata
Jan 29, 2003·The Journal of Nutritional Biochemistry·Wayne Y. EnsignMaria Luz Fernandez
Nov 13, 2004·The Journal of Nutritional Biochemistry·M Vergara-JimenezM L Fernandez
Dec 22, 1999·The Proceedings of the Nutrition Society·B de RoosL L Rudel
Mar 16, 2007·European Journal of Clinical Nutrition·K A GreanyM S Kurzer
Jan 13, 2000·Proceedings of the Society for Experimental Biology and Medicine·T P CarrC L Schneider
Feb 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anuradha RaoPaul A Dawson
Feb 11, 2003·The British Journal of Nutrition·Min ChenTapan K Basu
Jan 5, 2008·Photochemistry and Photobiology·Omer Yalinkilic, Huseyin Enginar
Dec 31, 2008·Molecular and Cellular Biology·Tayeba KhanPhilipp E Scherer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.