Enzymatic Evidence for a Complete Oxidative Pentose Phosphate Pathway in Chloroplasts and an Incomplete Pathway in the Cytosol of Spinach Leaves

Plant Physiology
C SchnarrenbergerW Martin


The intracellular localization of transaldolase, transketolase, ribose-5-phosphate isomerase, and ribulose-5-phosphate epimerase was reexamined in spinach (Spinacia oleracea L.) leaves. We found highly predominant if not exclusive localization of these enzyme activities in chloroplasts isolated by isopyknic centrifugation in sucrose gradients. Glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, glucose phosphate isomerase, and triose phosphate isomerase activity was present in the chloroplast fraction but showed additional activity in the cytosol (supernatant) fraction attributable to the cytosol-specific isoforms known to exist for these enzymes. Anion-exchange chromatography of proteins of crude extracts on diethylaminoethyl-Fractogel revealed only a single enzyme each for transaldolase, transketolase, ribose-5-phosphate isomerase, and ribulose-5-phosphate epimerase. The data indicate that chloroplasts of spinach leaf cells possess the complete complement of enzymes of the oxidative pentose phosphate path-way (OPPP), whereas the cytosol contains only the first two reactions, contrary to the widely held view that plants generally possess a cytosolic OPPP capable of cyclic function. The chloroplast enzymes tran...Continue Reading


May 19, 2001·European Journal of Biochemistry·Mark G PoolmanDavid A Fell
May 22, 2003·The Journal of Biological Chemistry·Jorg SchwenderYair Shachar-Hill
Nov 21, 2015·Journal of Experimental Botany·Thomas D Sharkey, Sean E Weise
Nov 1, 1996·Plant Molecular Biology·C P MoehsW R Belknap
May 7, 2004·European Journal of Biochemistry·Manuela PailChristian P Kubicek
Mar 11, 2006·Molecular Biology and Evolution·Jörn PetersenHenner Brinkmann
Feb 4, 2014·Antioxidants & Redox Signaling·Monica BalseraBob B Buchanan
Apr 18, 2019·Planta·Mathias BockwoldtKarsten Fischer
Apr 2, 2016·Scientifica·Álvaro D Fernández-Fernández, F J Corpas
Oct 24, 2019·Journal of Experimental Botany·Hannes LansingAntje Von Schaewen
Jun 18, 2005·The Plant Journal : for Cell and Molecular Biology·Maxime Caillau, W Paul Quick
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·H. E. Neuhaus, Michael J Emes
May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Judith ScharteAntje von Schaewen
Apr 26, 2007·Journal of Molecular Evolution·Steven RobbensYves Van de Peer
May 9, 2009·Plant, Cell & Environment·Doug K AllenYair Shachar-Hill
Jan 25, 2003·European Journal of Biochemistry·Mark G PoolmanChristine A Raines
Dec 29, 2020·Frontiers in Plant Science·Alyssa L PreiserThomas D Sharkey

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, as well as fevers and skin rashes. Here is the latest research on this disease.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.


Trichotillomania is an impulse control disorder characterized by the recurrent pulling of one's hair resulting in noticeable hair loss. Here are the latest discoveries in this field.

DDX3X Syndrome

DDX3X syndrome is caused by a spontaneous mutation at conception that primarily affects females due to its location on the X-chromosome. DDX3X syndrome has been linked to intellectual disabilities, seizures, autism, low muscle tone, brain abnormalities, and slower physical developments. Here is the latest research.