Enzymatic hydrolysis and ethanol fermentation of high dry matter wet-exploded wheat straw at low enzyme loading

Applied Biochemistry and Biotechnology
Tania I GeorgievaBirgitte K Ahring

Abstract

Wheat straw was pretreated by wet explosion using three different oxidizing agents (H(2)O(2), O(2), and air). The effect of the pretreatment was evaluated based on glucose and xylose liberated during enzymatic hydrolysis. The results showed that pretreatment with the use of O(2) as oxidizing agent was the most efficient in enhancing overall convertibility of the raw material to sugars and minimizing generation of furfural as a by-product. For scale-up of the process, high dry matter (DM) concentrations of 15-20% will be necessary. However, high DM hydrolysis and fermentation are limited by high viscosity of the material, higher inhibition of the enzymes, and fermenting microorganism. The wet-explosion pretreatment method enabled relatively high yields from both enzymatic hydrolysis and simultaneous saccharification and fermentation (SSF) to be obtained when performed on unwashed slurry with 14% DM and a low enzyme loading of 10 FPU/g cellulose in an industrial acceptable time frame of 96 h. Cellulose and hemicellulose conversion from enzymatic hydrolysis were 70 and 68%, respectively, and an overall ethanol yield from SSF was 68%.

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Citations

Jun 10, 2009·Biotechnology for Biofuels·Jan B KristensenHenning Jørgensen
Sep 24, 2009·International Journal of Biological Sciences·Mehdi DashtbanWensheng Qin
Dec 26, 2015·Biotechnology for Biofuels·Rodrigo Morales VeraRick Gustafson
Feb 24, 2012·Biotechnology and Bioengineering·Alicia A Modenbach, Sue E Nokes
Nov 6, 2012·Biotechnology and Bioengineering·Zhenhua RuanYan Liu
Jun 21, 2011·Enzyme Research·Gary BrodeurSubramanian Ramakrishnan
Nov 16, 2013·Trends in Biotechnology·Rakesh KoppramLisbeth Olsson
Aug 3, 2012·Bioresource Technology·Diwakar RanaBirgitte K Ahring
Nov 19, 2013·Applied Biochemistry and Biotechnology·Daniel LudwigSusanne Zibek
May 28, 2013·Applied Biochemistry and Biotechnology·S I NjokuH Uellendahl
Dec 15, 2020·FEMS Microbiology Reviews·Justyna Ruchala, Andriy A Sibirny

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