Enzymatic hydrolysis in an aqueous organic two-phase system using centrifugal partition chromatography

Journal of Chromatography. a
J KrauseJ Merz

Abstract

Multi-phase reaction systems, mostly aqueous organic systems, are used in enzyme catalysis to convert hydrophobic substrates which are almost insoluble in aqueous media. In this study, a Centrifugal Partition Chromatograph is used as a compact device for enzymatic multi-phase reaction that combines efficient substrate supply to the aqueous phase and separation of both phases in one apparatus. A process design procedure to systematically select the aqueous and organic phase to achieve stable and efficient reaction rates and operation conditions in Centrifugal Partition Chromatography for efficient mixing and separation of the phases is presented. The procedure is applied to the hydrolysis of 4-nitrophenyl palmitate with a lipase derived from Candida rugosa. It was found that the hydrolysis rate of 4-nitrophenyl palmitate was two times higher in Centrifugal Partition Chromatography than in comparable stirred tank reactor experiments.

References

Sep 1, 1993·Enzyme and Microbial Technology·H M Van SonsbeekJ Tramper
Aug 12, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·J L den HollanderL A van der Wielen
Jul 10, 2003·Angewandte Chemie·Murillo Villela FilhoChristian Wandrey
Jan 1, 1987·Biotechnology Advances·P J Halling
Nov 5, 2005·Journal of Chromatography. a·A Marston, K Hostettmann
Apr 28, 2006·Journal of Agricultural and Food Chemistry·Qixin Zhong, Charles E Glatz
Feb 27, 2007·Journal of Chromatography. a·J Brent Friesen, Guido F Pauli
Aug 12, 2008·Advances in Colloid and Interface Science·P ReisR Miller
Jan 13, 2009·Journal of Chromatography. a·J Brent Friesen, Guido F Pauli
Jan 19, 2010·Seikagaku. The Journal of Japanese Biochemical Society·Hiroyasu Ogino
Feb 18, 2011·Journal of Chromatography. a·S AdelmannG Schembecker

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Citations

Feb 1, 2017·Journal of Separation Science·Małgorzata BojczukPaweł Hodurek
Jan 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Radu Claudiu FierascuElwira Sieniawska

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