PMID: 6856Jun 1, 1976

Enzymatic hydrolysis of 1-monoacyl-SN-glycerol-3-phosphoryl-choline (1-lysolecithin) by phospholipases from peanut seeds

Lipids
H StraussM Heller

Abstract

Hydrolysis of 1-lysolecithin (1-acyl glycerophosphorylcholine [1-acyl GPC]) by preparations of phospholipase D from peanut seeds was investigated. 1-Lysolecithin was hydrolyzed at a much slower rate than phosphatidylcholine (lecithin). Although Ca+2 ions are required for the cleavage of lecithin by the enzyme, their effect on the hydrolysis of lysolecithin depended upon the concentration of the substrate: at 0.2 mM 1-lysolecithin, Ca+2 ions increased the reaction rates, whereas at concentrations of the substrate lower than 0.1 mM, Ca+2 ions were inhibitory. A broad pH activity curve between 5 and 8 was obtained with higher rates in the alkaline range, both in the absence and presence of Ca+2 ions. The increased hydrolysis of lysolecithin due to Ca+2 was noticed over the entire pH range. Upon storage of the enzyme solutions at 4 C, decreased rates of hydrolysis of lecithin were observed, with t 1/2 values of ca. 50 and 100 days depending on the purity of the preparation. During the same period, no reduction occurred in the activity of these preparations on lysolecithin as substrate. The effects of Ca+2 ions and the analysis of the products of 1-acyl GPC cleavage by the enzyme preparations revealed the presence of more than one e...Continue Reading

References

Oct 1, 1965·Canadian Journal of Biochemistry·H Brockerhoff, M Yurkowski
Jan 1, 1967·The Biochemical Journal·C LONGE J Sargent
Apr 1, 1956·The Biochemical Journal·R M DAWSON
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
May 1, 1963·Proceedings of the Society for Experimental Biology and Medicine·W M DOIZAKI, L ZIEVE

Citations

Apr 1, 1978·Journal of the American Oil Chemists' Society·R G JensenK E McMahon
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Xuemin Wang
Jan 20, 2016·Progress in Lipid Research·Yueyun HongXuemin Wang
Oct 24, 2012·Progress in Lipid Research·Igor PokotyloJan Martinec
May 3, 2015·Cell Calcium·Amarjeet SinghGirdhar K Pandey
Nov 5, 1997·Analytical Biochemistry·A J MorrisJ Engebrecht
Jun 12, 2019·NMR in Biomedicine·Kanchan SonkarKristine Glunde
Dec 25, 2004·The Journal of Biological Chemistry·Yuki NakamuraHiroyuki Ohta

Related Concepts

Calcium
Hydrogen-Ion Concentration
Lysophosphatidylcholines
Arachis
Phosphatidylcholines
Phospholipase

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