PMID: 137Oct 1, 1975

Enzymatic hydrolysis of agar: purification and characterization of neoagarobiose hydrolase and p-nitrophenyl alpha-galactoside hydrolase

Canadian Journal of Microbiology
D F Day, W Yaphe

Abstract

The mixture of polysaccharides in the gelling component of agar (agarose) is hydrolyzed to D-galactose and 3,6-anhydro-L-galactose by a series of hydrolytic enzymes obtained from Pseudomonas atlantica. The final degradative step in the pathway of agarose decomposition is the hydrolysis of the alpha-linkage in the dissaccharide neoagarobiose yielding D-galactose and 3,6-anhydro-L-galactose. Pseudomonas atlantica when grown on agar produces two specific enzymes, p-nitrophenyl alpha-galactose hydrolase and neoagarobiose hydrolase. The purification and partial characterization of both enzymes are presented.

Citations

Mar 22, 2006·Applied Microbiology and Biotechnology·Gurvan MichelWilliam Helbert
Apr 25, 2012·Applied Microbiology and Biotechnology·Won-Jae ChiSoon-Kwang Hong
Jun 9, 2001·Bioresource Technology·I HassairiB B Gupta
Jan 8, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Saeyoung LeeIn Geol Choi
Dec 9, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Aiming RenJiahai Zhou
Oct 20, 2005·Journal of Bioscience and Bioengineering·Hisashi SuzukiKeiichi Kawai
Oct 20, 2005·Journal of Bioscience and Bioengineering·Hisashi SuzukiKeiichi Kawai
Aug 11, 2015·Frontiers in Microbiology·Nabajit DasSukant Khurana
May 20, 1996·Biotechnology and Bioengineering·M S ShoichetS R Winn
Dec 1, 1983·European Journal of Biochemistry·L M MorriceF B Williamson
Oct 3, 1983·European Journal of Biochemistry·L M MorriceW F Long
Aug 8, 2006·Biotechnology Journal·Assunta GiordanoAntonio Trincone
Feb 22, 2011·Environmental Microbiology·Etienne RebuffetGurvan Michel
Sep 7, 2017·Acta Crystallographica. Section F, Structural Biology Communications·Saeyoung LeeIn Geol Choi
Feb 18, 2020·Frontiers in Bioengineering and Biotechnology·Chengcheng JiangXiangzhao Mao
Feb 10, 2020·Applied Microbiology and Biotechnology·Si Hyoung ParkSoon-Kwang Hong

Related Concepts

Agar
Ammonium Sulfate
Plasma Membrane
Cell-Free System
Exoglycosidases
Hydrogen-Ion Concentration
Hydrolysis
Lithium
Nitrophenylgalactosides
Polysaccharides

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