Abstract
Agricultural practices generate lignocellulosic waste that can be bioconverted by fungi to generate value-added products such as biofuels. In this context, fungal enzymes are presented as an alternative for their use in the hydrolysis of cellulose to sugars that can be fermented to ethanol. The aim of this work was to characterize LBM 033 strain and to analyze its efficiency in the hydrolysis of cellulosic substrates, including barley straw. LBM 033 strain was identified as Trametes villosa by molecular techniques, through the use of the ITS and rbp2 markers and the construction of phylogenetic trees. The cell-free supernatant of T. villosa LBM 033 showed high titers of hydrolytic enzymatic activities, necessary for the hydrolysis of the holocellulosic substrates, hydrolyzing pure cellulose to cellobiose and glucose and also degraded the polysaccharides contained in barley straw to short soluble oligosaccharides. These results indicate that macro fungi from tropical soil environments, such as T. villosa LBM 033 can be a valuable resource for in-house, cost effective production of enzymes that can be applied in the hydrolysis stage, which could reduce the total cost of bioethanol production.
References
Mar 29, 2001·Bioresource Technology·A S BallJ Robinson
Mar 2, 2005·Molecular Phylogenetics and Evolution·P Brandon Matheny
Mar 14, 2007·Bioresource Technology·Vladimir ElisashviliGiorgi Kvesitadze
Oct 24, 2007·Journal of Applied Microbiology·F GuglielmoM Garbelotto
Oct 7, 2008·Mycologia·Kozue SotomeMakoto Kakishima
Oct 29, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sarah K McMenaminChristopher K Wright
Jun 23, 2009·Bioresource Technology·Marimuthu JeyaJung-Kul Lee
Oct 31, 2009·New Biotechnology·Badal C Saha, Michael A Cotta
Oct 29, 2010·Journal of Innate Immunity·Russell F Doolittle
Nov 19, 2011·FEMS Microbiology Letters·Laurence Lesage-MeessenAnne Lomascolo
Mar 27, 2012·Biotechnology Advances·J S Van Dyk, B I Pletschke
Dec 5, 2012·Journal of Microbiological Methods·Yann MathieuMarc Buée
Oct 18, 2013·Biotechnology for Biofuels·Dongyang LiuQirong Shen
Jun 6, 2014·Mycologia·Alexis CarlsonDavid S Hibbett
Nov 20, 2014·Journal of the Science of Food and Agriculture·Md Mominul Islam SheikhJae Won Kim
Aug 20, 2015·Molecular Ecology Resources·Tomáš VětrovskýPetr Baldrian
Mar 3, 2018·Aerobiologia·Rafał OgórekDana Tančinová
Aug 29, 2018·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Wilton Soares CardosoJosé Humberto de Queiroz
Jan 28, 2019·Reviews on Environmental Health·Bodjui Olivier AboQunhui Wang