Enzymatic hydrolysis of cellulosic materials by Sclerotium rolfsii culture filtrate for sugar production

Canadian Journal of Microbiology
J G Shewale, J Sadana


The hydrolysis of purified celluloses (cotton, Avicel, Cellulose-123, Solka Floc SW40) and cellulosic wastes (rice straw, sugarcane bagasse, wood powders, paper factory effluents) by Sclerotium rolfsii CPC 142 culture filtrate was studied. Factors which effect saccharification such as pH, temperature, enzyme concentration, substrate concentration, produce inhibition, adsorption, and inactivation of enzyme and particle size were studied. Virtually no inhibition (less than 3%) of cellulose hydrolysis by the culture filtrate was observed by cellobiose and glucose up to 100 mg/mL. Filter paper degrading enzyme(s) (but neither carboxymethylcellulase nor beta-glucosidase) was adsorbed on cellulose. The n value in the S. rolfsii system was calculated to be 0.32 for Avicel P.H. 101 and 0.53 for alkali-treated (AT) rice straw indicating penetration of cellulase into AT rice straw. In batch experiments at 10% substrate level, solutions containing 6 to 7%, 3.8 to 4.7%, 4.0 to 5.1%, and 4.2 to 4.9% reducing sugars were produced in 24 to 48 from AT rice straw. AT bagasse, alkali - peracetic acid treated mesta wood and paper factory sedimented sludge effluent, respectively. The main constituent in the hydrolysate from cellulose was glucose w...Continue Reading


Mar 29, 2011·Applied Biochemistry and Biotechnology·Bernardo C VidalVijay Singh
Jan 1, 1982·The International Journal of Biochemistry·J G Shewale
Jan 1, 1989·Critical Reviews in Biotechnology·V S Bisaria, S Mishra
Mar 1, 1981·Archives of Biochemistry and Biophysics·J G Shewale, J Sadana

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