Enzymatic hydrolysis of ionic liquid-extracted chitin

Carbohydrate Polymers
Paula BertonRobin D Rogers

Abstract

Chitin, one of Nature's most abundant biopolymers, can be obtained by either traditional chemical pulping or by extraction using the ionic liquid (IL) 1-ethyl-3-methylimidazolium acetate. The IL extraction and coagulation process provides access to a unique chitin, with an open hydrated gel-like structure. Here, enzymatic hydrolysis of this chitin hydrogel, dried shrimp shell, chitin extracted from shrimp shells using IL and then dried, and commercial chitin was carried out using chitinase from Streptomyces griseus. The enzymatic hydrolysis of shrimp shells resulted only in the monomer N-acetylglucosamine, while much higher amounts of the dimer (N,N'-diacetylchitobiose) compared to the monomer were detected when using all forms of 'pure' chitin. Interestingly, small amounts of the trimer (N,N',N''-triacetylchitotriose) were also detected when the IL-chitin hydrogel was used as substrate. Altogether, our findings indicate that the product distribution and yield are highly dependent on the substrate selected for the reaction and its hydrated state.

References

Jul 3, 1998·Current Opinion in Biotechnology·R Cohen-Kupiec, I Chet
May 2, 2002·Journal of the American Chemical Society·Richard P SwatloskiRobin D Rogers
Aug 19, 2006·Biotechnology and Bioengineering·Anantharam P DadiConstance A Schall
Mar 1, 1949·The Journal of Physical and Colloid Chemistry·E E HARRIS, A A KLINE
Feb 1, 1985·Biotechnology and Bioengineering·M S Bertran, B E Dale
Sep 30, 2008·Journal of Biotechnology·Hua ZhaoVernecia N Person
Jan 26, 2010·Bioresource Technology·Indira P Samayam, Constance A Schall
Feb 13, 2010·The FEBS Journal·Mélanie HallAndreas S Bommarius
Apr 23, 2013·Biotechnology Progress·S B Chavan, M V Deshpande
Jun 21, 2013·Frontiers in Microbiology·Sara Beier, Stefan Bertilsson
Jun 12, 2014·Biotechnology for Biofuels·Nirmal UppugundlaVenkatesh Balan
Jul 7, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·J L ShamshinaR D Rogers

❮ Previous
Next ❯

Citations

Apr 3, 2020·FEMS Microbiology Letters·Daisuke NikiJiro Arima
Feb 6, 2020·Marine Drugs·Nathanael D ArnoldThomas B Brück
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Julia L Shamshina, Paula Berton

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.